肝细胞肝癌TAE前后血清HIF-1α、VEGF表达与疗效的相关性研究

Expression of HIF-1α and VEGF in hepatocellular carcinoma before and after transcatheter arterial embolization: a clinical study

目的:探讨肝细胞肝癌(HCC)经肝动脉栓塞(TAE)治疗前后血清乏氧诱导因子-1α(HIF-1α)、血管内皮生成因子(VEGF)的表达及其与疗效的相关性。方法:2015年1月至2015年12月首诊HCC并行TAE36例,分别在TAE前1 d、TAE后3 d和1个月采集血清并行酶联免疫吸附试验(ELISA)测定血清HIF-1α和VEGF。根据TAE治疗后1个月复查疗效结果,将HCC患者分为完全缓解组与部分缓解+疾病稳定+进展组。结果:TAE前1 d血清HIF-1α和VEGF值分别为(136.56±69.36)和(287.83±125.74)ρg·ml^(-1),TAE后3 d分别为(325.15±36.21)和(395.17±98.36)ρg·ml^(-1),均显著高于TAE治疗前(P<0.05)。TAE后1个月,完全缓解组血清HIF-1α和VEGF值均低于部分缓解+疾病稳定+进展组(P<0.05)。血清HIF-1α、VEGF表达与疗效呈正相关(r=0.69,P<0.05)。结论:HCC患者TAE后短期内血清HIF-1α与VEGF均呈高表达,TAE后1个月血清HIF-1α及VEGF表达与疗效呈正相关。

Objective： To investigate the expression levels of hypoxia inducible factor-1 alpha（ HIF-1α） and vascular endothelial growth factor（ VEGF） before and after transcatheter arterial embolization（ TAE） in hepatocellular carcinoma（ HCC）,and their correlations with therapeutic effects. Methods： Thirty-six consecutive patients fulfilling diagnostic criteria for HCC undergoing TAE from January 2015 to December 2015 were enrolled into this clinical study. The serum HIF-1α and VEGF levels of all patients were analyzed using enzyme-linked immunosorbent assay（ ELISA） 1 day before-TAE,and 3 days,1 month after-TAE. All patients were divided into complete response（ CR） and partial response（ PR）,stable disease（ SD）,progressive disease（ PD） groups according to the therapeutic efficacy after one month. Results： The expression levels of serum HIF-1α and VEGF in HCC patients were（ 136. 56 ± 69. 36） and（ 287. 83 ± 125. 74） ρg·ml-（- 1）before TAE respectively. Three days after TAE,both serum HIF-1α（ 325. 15 ± 36. 21） and VEGF levels（ 395. 17 ± 98. 36） ρg·ml-（- 1）reached the peak values,and were higher than those before TAE（ P〈0. 05）. The levels of HIF-1αand VEGF in CR group after one month were significantly lower than those in PR ＋ SD ＋ PD group（ P〈0. 05）. The level of serum HIF-1α was positively correlated with serum VEGF level（ r = 0. 69,P〈0. 05）. Conclusion： The serum levels of HIF-1α and VEGF were in high expression in short time after TAE in HCC. The expression of HIF-1α and VEGF was positively correlated with clinical effects.