鲁西黄牛MSTN基因上游序列多态性对转录表达的影响

Effect of MSTN Gene Upstream Sequence Polymorphism on Transcriptional Expression in Luxi Huang Cattle

肌肉生长抑制素(myostatin,MSTN)基因是转化生长因子-β(transforming growth factor-β,TGF-β)超家族的新成员。为了研究鲁西黄牛MSTN基因上游序列多态性与转录表达的关系,本试验提取鲁西黄牛腿肌组织细胞的基因组,扩增出MSTN基因上游序列,构建进化树并通过基因测序确定其上游序列中存在单核苷酸多态位点,多态位点位于起始密码子上游805bp处。构建表达载体,在体外对C2C12细胞进行转染,从而验证MSTN基因上游序列多态位点对转录的影响。结果显示,MSTN基因上游序列中单个核苷酸的改变会影响其下游基因的表达水平。推测在体内MSTN基因上游序列中单核苷酸多态性能够影响基因的转录活性,在调节基因转录的过程中起着重要作用。

Myostain （MSTN）gene is a member of transforming growth factor-β（TGF-β）super-family.In order to study the relationship between Luxi Huang cattle MSTN gene upstream se-quence polymorphism and the transcriptional expression,DNA was extracted from leg muscle cells of Luxi Huang cattle and then sequenced.After analyzing MSTN upstream sequence,an ev-olutionary tree was constructed and a SNP site was found at 805 bp of MSTN upstream sequence of initiation codon.Two expression vectors contained the SNP site were constructed to transfect the C2C12 cells invitro to study the influence of MSTN gene upstream sequence polymorphism on transcription activity.The results showed that single nucleotide change on MSTN gene upstream sequence affected the expression.It suggested that MSTN gene upstream sequence pol-ymorphism of Luxi Huang cattle would affect transcription activity and play an important role in gene expression regulation.