摘要
目的：探讨微RNA-224（miR-224）对前列腺癌细胞增殖、凋亡、侵袭及转移的影响。方法构建携带过表达miR-224的重组慢病毒载体，分别转染前列腺癌细胞株PC3和LNCaP为miR-224过表达组，以转染空白载体的PC3和LNCaP为空载体对照组。实时荧光定量PCR检测miR-224在各组细胞中的表达，应用细胞增殖实验、划痕实验、细胞侵袭实验和流式细胞术检测各组细胞的增殖、迁移、侵袭和凋亡，建立裸鼠皮下移植瘤模型并观察miR-224对肿瘤生长的影响。结果与空载体对照组比较，miR-224过表达组的miR-224表达均显著上调（均P〈0.01）。与空载体对照组比较，miR-224过表达组细胞增殖能力显著下降（均P〈0.05）。划痕实验显示miR-224显著抑制前列腺癌细胞迁移（均P〈0.01）。流式细胞术显示过表达miR-224促进前列腺癌细胞凋亡（均P〈0.01）。细胞侵袭实验显示miR-224表达升高减弱肿瘤细胞的侵袭能力（均P〈0.05）。裸鼠移植瘤实验表明miR-224表达升高抑制移植瘤生长。结论 miR-224在前列腺癌中具有肿瘤抑制作用。
Objective To investigate the effects of microRNA-224 （miRNA-224） on the proliferation,apoptosis,invasion and migration of prostate cancer. Methods The recombinant lentiviral vectors with overexpressed miR-224 were established to transfect prostate cancer cell lines PC3 and LNCaP （overexpressed miR-224 group）,and empty vectors to transfect PC3 and LNCaP （empty vector control group）. Real-time fluorescence quantitative PCR was used to determine the expression of miR-224 in each group. Using cell proliferation assay ,scratch assay ,cell invasion assay and flow cytometry to determine cell proliferation,migration,invasion and apoptosis in each group. The subcutaneous tumor xenograft models in nude mice were established ,and the effects of miR-224 on tumor growth were determined. Results Compared with the empty vector control group,the miR-224 expression was significantly increased （both P〈0.01）and the cell proliferation was significantly decreased（all P〈0.05）in the overexpressed miR-224 group. Scratch assay showed that miR-224 significantly inhibited prostate cancer cell migration（both P〈0.01）. Flow cytometry showed that overexpressed miR-224 promoted prostate cancer cell apoptosis（both P〈0.01）. Cell invasion assay showed that the increased expression of miR-224 decreased the invasion of tumor cells （both P〈0.05）. The xenograft tumor of prostate cancer cell in nude mice showed that the increased expression of miR-224 inhibited the growth of xenograft tumor. Conclusion MiR-224 plays a role of tumor inhibition in prostate cancer.
出处
《中华生物医学工程杂志》
CAS
2016年第1期7-11,共5页
Chinese Journal of Biomedical Engineering
