含锌活性涂层种植体材料对细菌黏附的影响

Effect of active Zinc-containing coatings against bacterial adherence on surface of denture implant ;materials

目的：探索纯钛种植体材料表面含锌（Zn）活性涂层化学组成及对细菌黏附能力的影响。方法应用等离子体电解氧化技术，在纯钛表面制备含Zn的活性涂层，分为低锌组（L-Zn）、中锌组（M-Zn）和高锌组（H-Zn）、阴性对照组（不含Zn）。X射线光电子能谱分析涂层化学组成及各元素百分比；荧光法评价4组涂层材料对变形链球菌（Streptococcus mutans，S.m）黏附的影响；场发射扫描电镜观察细菌在涂层表面黏附数量和菌体形态的变化。结果 X线光电子能谱（X-ray photoelectron spectroscopy，XPS）分析显示随着电解液中锌浓度的提高，涂层中锌所占比重随之增加；荧光法实验表明，与对照组（30.53±9.33）比较，各实验组（L-Zn组：20.93±6.13；M-Zn组：19.47±7.35；H-Zn组：16.87±5.38）涂层表面S.m黏附的数量均明显减少（P〈0.05）；电镜示随锌含量的升高涂层表面细菌黏附数量逐渐减少。结论含锌活性涂层可有效抑制S.m黏附并影响其菌体形态，且与锌的含量有关。

Objective To explore the effects of chemical composition with active zinc （Zn）-containing coatings on pure titanium implant surface against bacterial adherence. Methods Plasma electrolytic oxidation（PEO）was applied to prepare active Zn-containing coatings on pure titanium implant surface. The implants were then divided into four groups：low zinc group（L-Zn）,medium zinc group（M-Zn）,high zinc group （H-Zn）,and the negative control group （without Zn）. X-ray photoelectron spectroscopy（XPS）was used to analyze the chemical composition and percentages of the various elements in the coatings. Fluorescence method was used to evaluate the adherence of Streptococcus mutans（S mutans） on the surface of four groups. Field scanning electron microscopy（FSEM）was used to observe the changes in the number and morphology of S mutans on the coating surface. Results XPS analysis showed that,along with the increasing zinc concentration in the electrolyte,the proportion of zinc in the coatings increased. Fluorescence experiments showed,compared with the control group（30.53 ± 9.33）,there were significantly lower level of S mutans adhesion on the coating surface of experimental groups（L-Zn group：20.93±6.13;M-Zn group：19.47 ± 7.35;H-Zn group：16.87 ± 5.38）（all P〈0.05）. FSEM showed that the number of surface-adhering bacteria gradually decreased with higher content of zinc in the coatings. Conclusion Active Zn-containing coating can effectively inhibit adhesion of S mutans and affect the bacterial morphology. This effect associated with zinc content.