RANTES基因多态性与子痫前期关系的研究

Relationship between RANTES gene polymorphism and pre - eclampsia

目的：探讨孕妇调节活化正常T细胞表达与分泌的趋化因子（regulated on activation normal T cell expressed and secreted ，RANTES）基因多态性是否影响子痫前期（pre-eclampsia，PE）的发生及其可能机制。方法利用聚合酶链反应-限制性内切酶片段长度多态性（PCR-RFLP）方法对2012年8月-2015年8月于本院住院治疗的120例PE孕妇（PE组）和200例健康对照组孕妇的RANTES基因进行多态性（SNPs）分析，Western blotting法检测两组孕妇胎盘RANTES含量。结果 RANTES-403， RANTES-28各等位基因型分布在PE组和对照组间分布差异无统计学意义（P＆gt;0.05），PE组胎盘RANTES表达指数高于正常组（0.736±0.249 vs 0.468±0.15，P〈0.05）。PE组RANTES表达指数在基因型为RANTES-403A/A时较RANTES-403G/G时明显减少（0.429±0.107 vs 0.743±0.132，P〈0.05）。结论 RANTES在PE患者胎盘表达增加，可能参与PE发生，RANTES基因多态性可能影响RANTES蛋白水平的高低。

Objective To investigate whether the cytokine regulated upon activation normal T cell expressed and secreted （RANTES） genetic polymorphisms affects occurrence of pre-eclampsia （PE） in pregnant women and its possible mechanism. Methods Using techniques of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）,we studied the single nucleotide polymorphism （SNP）of RANTES in 120 pregnant women with PE（PE group）and 200 healthy pregnant women（control group）in our hospital between August 2012 and August 2015. Western blotting were used to detect RANTES level in placenta. Results There was no significant difference in allele distribution frequency of RANTES-403 and RANTES-28 between the PE and control groups（P＆gt;0.05）. The placenta RANTES gene expression index was higher in the PE group than in the healthy control group（0.736±0.249 vs 0.468±0.15,P〈0.05）. In the PE group,significantly lowered expression index of RANTES was seen in subjects with genotype RANTES-403A/A compared to those with genotype RANTES-403 G/G（0.429 ± 0.107 vs 0.743 ± 0.132,P〈0.05）. Conclusion RANTES expression was enhanced in placenta of patients with PE,suggesting that it may be involved in occurrence of PE. RANTES single nucleotide polymorphism may be associated with the level of RANTES protein production.