HPLC测定柴葛清热颗粒中柴胡皂苷a、葛根素和黄芩苷含量

Determination of Saikosaponin a, Paeoniflorin and Baicalin in Chaigeqingre Granula by HPLC

目的：建立柴葛清热颗粒中柴胡皂苷a、葛根素和黄芩苷含量的测定方法。方法采用HPLC法，色谱柱为Agilent-C18色谱柱（150 mm×4.6 mm，5μm），流动相：0.1﹪磷酸水溶液（A）-乙腈（B），梯度洗脱（0-12 min，15﹪-20﹪ B；12-30 min，20﹪-25﹪ B），流速为1.0 mL·min-1，检测波长210 nm，柱温30℃，进样量20μL。结果胡皂苷a在1.96-31.36μg·mL-1，葛根素在9.4-150.4μg·mL-1，黄芩苷1.52-24.32μg·mL-1范围内线性关系良好。平均回收率分别为97.65﹪、103.56﹪、102.83﹪，RSD分别为1.29﹪、1.14﹪、1.20﹪。结论该方法准确简便重复性好，可用于柴葛清热颗粒中柴胡皂苷a、葛根素和黄芩苷的含量测定。

Objective To establish a method for the determination of saikosaponin a, paeoniflorin and baicalin in Chaigeqingre granula by HPLC.Methods The chromatographic column was Agilent-C18（150 mm×4.6 mm, 5 μm）, using the gradient mobile phase composed of 0.1% H3PO4 （A） -acetonitrile （B）, （0-12 min, 15-20% B; 12-30 min, 20-25% B）, the lfow rate was 1.0 mL·min-1 and the detection wavelength was 210 nm. The temperature of column was 30℃, and the injection volume was 20 μL.Results The linear range of saikosaponin a, paeonilforin and baicalin was 1.96-31.36 μg·mL-1, 9.4-150.4 μg·mL-1 and 1.52-24.32 μg·mL-1 with average recovery of 97.65% （RSD=1.29%）, 103.56% （RSD=1.14%）, 102.83% （RSD=1.20%）.Conclusion The method is convenient, accurate and reproducible for the determination of saikosaponin a, paeonilforin and baicalin in Chaigeqingre granula.