山药地上部分石油醚部位的化学成分研究

Chemical constituents of the petroleum ether fraction from the aerial part of Dioscorea opposita

目的研究山药地上部分石油醚部位的化学成分。方法采用硅胶、Sephadex LH-20等多种柱色谱方法对山药地上部分的石油醚部位进行分离纯化,并通过波谱数据分析（核磁共振谱和质谱）进行结构鉴定。结果从山药地上部分石油醚部位中共分离鉴定了11个化合物,分别为9,19-cyclolart-25-en-3β,24R-diol（1）、cycloeucalenol（2）、表木栓醇（3）、（24S）-24-乙基胆甾-3β,5α,6β-三醇（4）、豆甾-4-烯-3α,6β-二醇（5）、（22E）-5α,8α-epidioxyergosta-6,22-dien-3β-ol（6）、（3β,7α）-7-methoxystigmast-5-en-3-ol（7）、β-谷甾醇（8）、1-正十六烷酸甘油酯（9）、二十四烷酸（10）、十六烷酸（11）。其中1-3为三萜类,4-8为甾醇类,9-11为有机酸及其酯类。结论化合物1-5和7为首次从该属植物中分离得到,化合物1-7和9-10为首次从该植物中分离得到。

Objective To investigate the chemical constituents of the petroleum ether fraction from the aerial part of Dioscorea opposita. Methods Compounds were isolated and purified by various column chromatographic methods including silica gel and Sephadex LH-20 column chromatographies. Their structures were elucidated on the basis of spectral analysis（ nuclear magnetic resonance and mass spectrum）. Results Eleven compounds were isolated from the petroleum ether fraction of the aerial part of Dioscorea opposita as 9,19-cyclolart-25-en-3β,24R-diol（ 1）,cycloeucalenol（ 2）,epifriedelanol（ 3）,（ 24S）-24-ethylcholsta-3β,5α,6β-triol（ 4）,stigmast-4-en-3α,6β-diol（ 5）,（ 22E）-5α,8α- epidioxyergosta-6,22-dien-3β-ol（ 6）,（ 3β,7α）-7-methoxystigmast-5-en-3-ol（ 7）,β-sitosterol（ 8）,hexadecanoic acid 2,3-dihydroxy-propyl ester（ 9）,tetracosanoic acid（ 10）,palmitic acid（ 11）,respectively. Conclusion Compounds 1- 5 and 7 were isolated from the genus Dioscorea for the first time. Furthermore,compounds 1- 7 and 9- 10 have not been recorded before in this plant.