加味痛泻要方对肝郁脾虚型肠易激综合征患者小肠黏膜5-羟色胺含量及其受体mRNA表达的影响

Influence of Jiawei Tongxie decoction to recipe on liver stagnation and spleen deficiency type of irritable bowel syndrome patients with small intestinal mucosal 5-HT and its receptor mRNA expression

[目的]探讨加味痛泻要方治疗肝郁脾虚腹泻型肠易激综合征(IBS)患者的小肠黏膜5-羟色胺(5-HT)含量及其受体mRNA表达的影响。[方法]将60例肝郁脾虚IBS患者采取随机方法分为治疗组、对照组各30例,治疗组予加味痛泻要方汤剂口服治疗,对照组给予匹维溴铵片口服,疗程均为4周。观察2组患者治疗前后小肠黏膜5-HT含量及其受体mRNA表达水平的变化。[结果]1治疗组、对照组给药前后患者临床症状积分差异有统计学意义,且治疗组在食欲不振、倦怠乏力等方面改善更为显著(P<0.05);2治疗组、对照组给药前后血清5-HT含量差异有统计学意义(P<0.05),与对照组比较差异无统计学意义;3治疗组治疗后结肠黏膜5-HT含量及5-HT3、5-HT4受体mRNA的表达与治疗前比较均明显降低,二者比较差异有统计学意义;与对照组比较差异无统计学意义。[结论]加味痛泻要方能显著改善肝郁脾虚腹泻型IBS患者的的临床症状,其作用机制可能通过降低血清小肠黏膜5-HT含量及降低5-HT受体mRNA表达降低而实现。

[Objective]：To investigate the effect of Jiawei Tongxie decoction in the treatment of liver stagnation and spleen deficiency diarrhea type irritable bowel syndrome（IBS）patients with intestinal mucosal 5-HT and its receptor mRNA expression.[Methods]60cases of stagnation of liver Qi with deficiency of the spleen IBS patients were randomly assigned into the treatment group and the control group,30 cases in each group,the treatment group were given Jiawei Tongxie decoction oral treatment,while the control group were treated with pinaverium bromide tablets,the course of the two groups is 4weeks.Then observe the changes of 5-expression level and mRNA expression in the small intestine mucosa of the two groups before and after treatment.[Results]The clinical symptomatic score of the treatment group and the control group before and after the treatment have significant differences,and the treatment group in terms of loss of appetite,fatigue was improved more significant（P〈0.05）;②Both in the treatment group and the control group,5-hydroxy tryptamine content in the blood serum have significant difference（P〈0.05）before and after administration,but compares the non-statistics difference with the pinaverium bromide group;③Compared with the colon mucous membrane 5-HT content and 5-HT3,5-HT4 acceptor mRNA expression before treatment,which obviously reduces after therapy,the comparison has statistics difference,but compares the non-statistics difference with the pinaverium bromide group.[Conclusion]Jiawei Tongxie decoc-tion are able obviously to improve the IBS（stagnation of liver Qi with deficiency of the spleen）patients＇ s clinical symptoms,its function mechanism is possibly related with down-regulation of 5-hydroxy tryptamine content and 5-hydroxy tryptamine acceptor mRNA expression in the blood serum and on the small intestine mucous.