CD36mRNA在卵巢颗粒细胞的表达调节及与多囊卵巢综合征的相关性

Expression and regulation of CD36 mRNA in granulosa cells and its relation with clinical characteristics of polycystic ovary syndrome

目的 探讨CD36mRNA在多囊卵巢综合征（PCOS）患者卵巢颗粒细胞的表达,以及不同浓度的睾酮、胰岛素及罗格列酮对正常卵巢颗粒细胞CD36mRNA的表达调节.方法 分别提取5例PCOS患者和5名健康人卵巢颗粒细胞,应用实时荧光定量RT-PCR方法检测CD36mRNA的表达;提取25名健康人卵巢颗粒细胞进行体外培养48 h后,分别用含不同浓度的睾酮、胰岛素及PPARγ的激动剂（罗格列酮）的培养基培养颗粒细胞24 h后,应用RT-PCR方法检测CD36mRNA的表达.结果 （1）PCOS组颗粒细胞CD36 mRNA表达明显高于对照组（P＜0.01）.（2）颗粒细胞体外培养,当睾酮为1 nmol/L时,CD36 mRNA表达增加,但差异无统计学意义（P＞0.05）;当睾酮增加到10 nmol/L时,CD36 mRNA表达明显高于对照组（P＜0.05）.胰岛素为10 nmol/L时,CD36mRNA表达较对照组增加,但差异无统计学意义（P＞0.05）;当胰岛素进一步增加到100 nmol/L时,CD36mRNA表达较对照组明显增加（P=0.05）.罗格列酮为1 nmol/L时,CD36 mRNA表达较对照组明显增加（P＜0.05）;当罗格列酮增加为10 nmol/L时,CD36 mRNA表达较罗格列酮为l nmol/L时进一步明显增加（P＜0.05）.结论 （1）卵巢颗粒细胞CD36 mRNA表达可能与PCOS高雄激素血症有关;（2）高浓度的睾酮和胰岛素诱导卵巢颗粒细胞CD36 mRNA表达;（3） CD36 mRNA表达随着罗格列酮浓度的增加而增加,因此颗粒细胞CD36 mRNA表达可能受PPAR的正向调节.

Objective To study the expression of CD36 mRNA in granulosa cells （GCs） of patients with polycystic ovary syndrome （PCOS） and the impact of testosterone,insulin and PPARγ agonist rosiglitazone on GCs.Methods The expression of CD36 mRNA inGCs of patients with PCOS and normal controls were assayed byreal-time PCR.The level of CD36 mRNA after treatment with testosterone,insulin,and rosiglitazone in GCs ofnormal controls were also tested by real-time PCR.Results （1） The expression of CD36mRNA in the GCs of PCOS was significantly higher than that of the controls （P 〈 0.05）.（2） When testosterone concentration was 1 nmol/L,CD36 mRNA increased in the GCs,but there was no significantdifference compared to the blank control,（P 〉 0.05）.When testosterone concentration was l0 nmol/L,the expression of CD36 mRNA in the GCs was higher than that in the blank control with significant difference （P 〈 0.05）.When insulin concentration was 10 nmol/L,the expression of CD36 mRNA increased but the difference was not statistically significant （P 〉 0.05）.When insulin concentration was 100 nmol/L,the expression of CD36mRNA in the GCswas higher than that in the blank control （P =0.05）.When rosiglitazone concentration was I nmol/L,the expression of PPARγ mRNA in GCs were significantly increased compared with the blank control （P 〈 0.05）.The expression of CD36 mRNA atrosiglitazone concentrationof 10 nmol/Lwere significantly increased compared to the concentration of 1 nmol/L （P 〈 0.05）.Conclusion High testosterone and insulin induced the expression of CD36 mRNA.Rosiglitazone increased CD36 mRNA in a dose-related manner in GCs.Increased CD36 mRNA in the GCs of PCOS may be related to the clinical characteristics of PCOS.