高危型人乳头瘤病毒感染患者阴道乳酸杆菌的变化

The change of vaginal lactobacillus in patients with high-risk human papillomavirus infection

目的 探讨人乳头瘤高危型病毒（HPV16/18）感染患者阴道乳酸杆菌菌种的分布特点.方法 显微油镜观察95例HPV16/18感染患者及90例体检的阴道微生态正常的育龄期女性阴道黏膜分泌物中乳酸杆菌的定植密度,并采用种特异性聚合酶链反应法检测两组阴道乳杆菌的菌种,了解HPV16/18感染患者阴道乳酸杆菌的分布特点.结果 HPV16/18感染患者阴道黏膜乳酸杆菌的定植密度为104（68 ～186）个/HP,低于正常对照组234 （161 ～326）个/HP（P＜0.05）;HPV感染组阴道内卷曲乳酸杆菌、惰性乳酸杆菌、加氏乳酸杆菌明显减少（P＜0.05）.结论 HPV16/18感染可能与阴道黏膜乳酸杆菌减少、阴道菌群失调相关;卷曲乳酸杆菌、惰性乳酸杆菌、加氏乳酸杆菌可能在维持阴道微生态环境中起主要作用.

Objective To study the distribution characteristics of lactobacillus in the vaginal mucosa of patients with HPV infection.Methods The planting density of lactobacillus in vaginal secretions of 95 cases with HPV16/18 infection and 90 cases of normal women of childbearing age were observed by oil microscope.And the strains of vaginal lactobacilli in two groups were analyzed using species-specific polymerase chain reaction （Species-specific PCR） and the distribution of vaginal lactobacilli in patients with HPV16/18 infection were investigated.Results In HPV16/18 infective groups,the planting density of lactobacillus in the vaginal mucosa was 104 （68-186）/HP.It was significantly lower than that of the normal group （234 （161-326）/HP,P 〈0.05）.Compared with the normal group,the positive rates of lactobacillus iners,lactobacillus crispatus,and lactobacillus gasseri were significantly lower in HPV16/18 infection group （P 〈 0.05）.Conclusion The HPV16/18 infection is associated with the decreased number of lactobacillus and the imbalance of vaginal flora;Lactobacillus iners,lactobacillus crispatus,and lactobacillus gasseri may play a key role in maintaining the vaginal micro ecological environment.