小鼠肝癌合并肝纤维化模型的构建——四氯化碳和二乙基亚硝胺诱导

Establishing a Mice Model Fhepatic Fibrosis Induced by the CCL_4 and DEN

研究四氯化碳联合二乙基亚硝胺诱导小鼠肝癌合并肝纤维化模型的建立。选小鼠24只,随机分为对照组与实验组。实验组每周两次腹腔注射15%的CCl4/花生油溶液,同时自由饮用1‰的二乙基亚硝胺(DEN)连续进行8周。对照组注射花生油,饮用蒸馏水。于处理后第3、6、8周处死实验组及对照组小鼠各4只,对其肝脏组织进行常规固定、包埋、切片、HE染色,观察肝脏的形态及变化。对照组与实验组小鼠体重差异显著(P<0.05),四氯化碳和二乙基亚硝胺能显著改变肝脏形态,使肝脏胶原纤维明显增生,肝星状细胞数量增加,细胞变长并分泌胶原,最终导致肝癌的形成。CCl4联合DEN诱导可成功建立小鼠肝癌并合并肝纤维化的模型。

The mice is taken as research materials to study the liver cancer and liver fibrosis in them when they were injected CCl4 and DEN at the same time. Kunming mouse were randomly divided into two groups. The experimental groups were injected by15% CCl4/ peanut oil solution intraperitoneally two times a week for eight weeks,meanwhile,drinking 1‰ DEN water solution freely. The control group was injected peanutoil,drinking purified water. Both experimental group and control group were killed at the 3,6 and 8 weeks respectively and were stained by HE dyeing,to observe the changes of liver structure and the number of sperm. The results show that the CCl4 and DEN can significantly change the liver morphology. In the end,the liver collagen fiber was obviously proliferated,the amount of hepatic stellate cells were increased,and cells became longer and secreted collagen,which led the liver to become fibrosis and eventually caused the formation of liver cancer. The results showed that the CCl4 and DEN can significantly change the liver morphology.