丙型肝炎病毒感染患者血清白细胞介素水平与肝功能相关性研究

Correlation between IL-2,IL-6 and liver function,hepatitis C virus RNA in the patients with hepatitis C virus infection

目的分析研究丙型肝炎患者白细胞介素-2(IL-2)、IL-6水平与肝功能及HCV-RNA含量的相关性。方法选取2014年1月-2015年9月于医院就诊的57例丙型肝炎患者作为研究对象,按临床情况分为无明显临床症状组23例,慢性丙型肝炎中重度组19例,重症肝炎肝硬化组15例,另选28名健康志愿者为对照组,抽取受试者空腹静脉血,检测肝功能及血清中IL-2、IL-6水平,采用荧光定量逆转录聚合酶链式反应检测HCV-RNA水平。结果无明显临床症状组患者血清中IL-2及Il-6细胞因子水平与正常对照组比较差异无统计学意义;但IL-2在中重度组和重症肝炎肝硬化组患者中的水平与对照组比较有显著性下降(P<0.05),Il-6在中重度组和肝硬化组中的水平与正常对照组比较有明显上升(P<0.05);丙型肝炎组患者的ALT、AST、TBIL和DBIL水平显著高于正常对照组(P<0.05);血清中IL-2及IL-6含量与HCV-RNA的含量无显著相关性。结论丙型肝炎患者随病情加重血清中IL-2水平逐渐增高、IL-6水平逐渐降低,HCV-RNA的拷贝数与疾病严重程度并无显著相关性。

OBJECTIVE To explore the correlation between IL-2,IL-6and liver function,hepatitis C virus RNA levels and liver function.METHODS From Jan.2014 to Sep.2015,57 patients with hepatitis C in our hospital were chosen as study objects.They were divided into no obvious clinical symptom group with 23 cases,moderate severe group with 19 cases and severe group with 15 cases according to clinical condition.Totally 28 healthy people were selected as control group.Fasting blood of the three groups of patients were collected to test liver function and serum IL-2and IL-6levels.Fluorogenic probe quantitative reverse transcription polymerase chain reaction was conducted to detect HCV-RNA level.RESULTS Patients without significant symptoms had similar serum IL-2and Il-6levels to the control group.The differences had no significantly significance（P〉0.05）.But serum IL-2contents of patients with mild and severe liver cirrhosis infection were notably lower than the control group（P〈0.05）;the serum IL-6contents of patients with moderate and severe liver cirrhosis were significantly higher than patients in the control group（P〈0.05）.ALT,AST,TBIL and DBIL levels of patients with hepatitis C were significantly higher than the normal control group（P〈0.05）.Serum IL-2and IL-6levels were not significantly correlated to HCV-ENA.CONCLUSION As hepatitis C worsen,serum IL-2levels increase and IL-6decrease.The condition of the disease have no correlation with HCV-RNA level.