甘蓝型油菜紫色酸性磷酸酶基因类似物的生物信息学分析

Bioinformatics Analysis of the PAPGene Analogs in Brassica napus

利用生物信息网络数据库,对甘蓝型油菜紫色酸性磷酸酶基因(PAP)类似物编码的蛋白进行生物信息学分析,以预测该蛋白的理化性质、分子结构与生物学功能。结果表明:该基因编码的蛋白含481个氨基酸,其蛋白的分子式为C2520H3714N666O719S19,分子量为55.45kDa,等电点为6.35,为亲水性分泌型蛋白,并且不稳定;存在3个跨膜螺旋区域及3个卷曲螺旋结构,含有1个信号肽、39个潜在磷酸化位点和3个N-糖基化修饰,存在6种类型特定的功能位点,主要定位于高尔基体;氨基酸序列同源性分析表明,该基因编码的蛋白与甘蓝型油菜PAP12家族具有较近的亲缘关系,直系同源于甘蓝型油菜PAP12-5;二级结构主要为无规则卷曲、延伸链和α?螺旋,具有较为明显的10个螺旋和26个折叠;具有PAP的保守结构域,属于金属磷酸二酯酶超家族成员;根据保守结构域及功能位点分析发现该基因属于紫色酸性磷酸酶类,可能属于甘蓝型油菜PAP基因家族成员,预测其编码产物在植物磷高效利用与吸收中发挥重要的生理功能。生物信息学分析及结合蛋白的结构与功能预测分析可为深入研究甘蓝型油菜PAP提供理论指导。

Bioinformatics network database and softwares were used to analyse and predict the biological properties of the PAP（purple acid phosphatase）gene analogs encoding protein in Brassica napus.The physical and chemical properties,structure and function of the protein were predicted,in the cause of providing a theoretical foundation for the further study on the adjustment mechanism of phosphorus absorption and utilization of Brassica napus.The results indicated that the gene encoded a length of 481 amino acid protein.The molecular formular was C2520H3714N666O719S19.Its molecular weight was 55.45 kDa and the PI was 6.35.It was predicted that the protein was hydrophilic,secretary protein and instability,and there was a signal peptide.The protein had 3transmembrane helical regions,3coiled coil structures,39 potential phosphorylation sites and 3N-glycosylated sites.It was mainly located in the Golgi apparatus.Homologous analysis showed that the gene had the close genetic relationship with Brassica napus PAP12,and orthologous to PAP12-5.Random coil,extend chains andα？helix were the main secondary strructions of the protein,and it had obvious10 helices and 26 folds.The protein had PAP＇s conserved domains,which belonged to metal phosphodiesterase super family（MPP）.The conserved domain analysis showed that it had two conserved motifs,seven metal-coordinating residues and six specific type functional sites,which predicted that the gene belonged to Brassica napus PAP gene family.Bioinformatics analysis and the protein structure and function prediction could provide theoretical guidance for further research on Brassica napus PAP.