“赤霞珠”葡萄组培快繁体系研究

Study on Tissue Culture and Rapid Propagation System of‘Cabernet Sauvignon'Grape

以"赤霞珠"葡萄为试材,对组培快繁中的取材部位、消毒方法、不同激素用量、是否带叶继代培养等关键技术措施进行了研究探讨。结果表明:以半木质化,0.1%升汞溶液消毒8min的茎段接种,成活率高,污染率低;并且继代培养和生根培养最好的培养基为1/2MS+IBA0.2mg·L^(-1)+蔗糖20g·L^(-1)+琼脂7g·L^(-1)。

Taking ‘Cabernet Sauvignon＇ grape as test material,on the tissue culture and rapid propagation of sampling location, disinfection methods,different hormone dosage, leaves subculture of key technical measures were discussed. The results showed that the semi lignified,0. 1% HgClz disinfection solution for 8 min of stems were inoculated, the survival rate was high,low pollution rate;and subculture and rooting culture medium for the best was 1/2MS＋IBA0.2mg·L-1＋sucrose 20 g · L-1 q-agar 7 g · L-1.