长白忍冬的组织培养与快速繁殖

Tissue Culture and Rapid Propagation of Lonicera ruprechtiana Regel

以长白忍冬的幼嫩茎段为外植体,MS为基本培养基,添加不同的植物生长调节物质,进行离体快速繁殖技术研究。结果表明:在0.1%HgCl_2溶液中滴加0.1%吐温80处理5min的灭菌效果最好;诱导长白忍冬侧芽分化的适宜培养基为MS+1.0mg·L^(-1) 6-BA+0.05mg·L^(-1) NAA+30g·L^(-1)蔗糖+7g·L^(-1)琼脂或不加任何生长调节物质的MS+30g·L^(-1)蔗糖+7g·L^(-1)琼脂;增殖培养基为MS+1.0mg·L^(-1) 6-BA+0.05mg·L^(-1) NAA+30g·L^(-1)蔗糖+7g·L^(-1)琼脂,30d的增殖系数为8;最佳的生根培养基为1/2MS+15g·L^(-1)蔗糖+7g·L^(-1)琼脂,生根率达60.0%。

Taking tender stem segments of Lonicera ruprechtiana Regel as explants,MS was selected as the basic medium supplemented with different plant growth regulators,the rapid propagation was studied.The results showed that the surface sterilization in 0.1% HgCl_2 solution adding 0.1% Tween 80 for 5min was the best.The optimal medium for inducing axillary buds was MS＋1.0mg·L（-1） 6-BA＋0.05mg·L（-1） NAA＋30g·L（-1） sucrose＋7g·L（-1） agar or MS＋30g·L（-1） sucrose＋7g·L（-1） agar;the medium MS＋1.0mg·L（-1） 6-BA＋0.05mg·L（-1） NAA＋30g·L（-1）sucrose＋7g·L（-1） agar was good for multiplication and the proliferation coefficient was 8.The optimum medium for root induction was 1/2MS＋15g·L（-1） sucrose＋7g·L（-1） agar,and the rooting rate was 60.0%.