摘要
病毒诱导基因沉默(virus-induced gene silencing,VIGS)技术,具有周期短、成本低、不需要转化遗传、高通量等优势,而被广泛地应用于探究植物生长发育、抗病及生理代谢等相关基因功能的鉴定。观赏植物具有较高的经济效益,VIGS技术在观赏植物中的应用具有良好的前景。现通过综述VIGS技术的建立与发展、作用机制及在观赏植物基因功能鉴定的应用实例,探讨该技术应用于观赏植物中影响沉默效率的因素;并展望其在观赏植物保护、丰富观赏性状的分子育种领域方面的应用前景。
Virus-induced gene silencing（VIGS）technolgy is widely used in identifying the functions of genes related to plant growth and development,resistance,metabolism and so on.VIGS technolgy has many merits,such as short period,low cost,no need for genetic transformation,high throughput.Ornamental plants have higher economical values,and it is expected VIGS technolgy can be widely used in them.This paper reviewed the mechanism,establishment and development of VIGS technolgy,factors affecting the efficiency of VIGS technolgy in ornamental plants,and applications of VIGS technolgy to ornamental plants.In addition,the prospects of VIGS technolgy in the applications to conservation and molecular breeding in ornamental plants were also discussed.
出处
《北方园艺》
CAS
北大核心
2016年第13期196-201,共6页
Northern Horticulture
基金
广东省科技厅星火计划资助项目(2012A020602101)
广东省科技计划资助项目(2013B020302006)
