siRNA沉默NCOA2基因对猪肌内前体脂肪细胞分化的影响

Influence of silencing NCOA2 gene by siRNA on differentiation of porcine intramuscular preadipocytes

[目的]本文旨在研究核受体共激活蛋白2(nuclear receptor coactivator 2,NCOA2)对猪肌内前体脂肪细胞分化能力的影响。[方法]用荧光定量PCR(qRT-PCR)法检测NCOA2在猪肌内前体脂肪细胞分化过程中的表达变化;通过NCOA2小干扰RNA(small interfering RNA,siRNA)抑制内源NCOA2的表达,并用油红O染色检测沉默NCOA2对肌内前体脂肪细胞分化能力的影响,通过qRT-PCR检测沉默NCOA2对脂肪分化关键基因过氧化物酶体增殖物激活受体γ(peroxisome proliferator activated receptorγ,PPARγ)、脂肪酸结合蛋白4(fatty acid binding protein 4,FABP4)、脂蛋白酯酶(lipoprotein lipase,LPL)和脂联素(adiponectin,ADIPOQ)表达的影响。[结果]NCOA2在肌内前体脂肪细胞分化第4天表达量显著升高(P<0.05);降低NCOA2表达可显著抑制肌内前体脂肪细胞分化(P<0.05),且PPARγ、FABP4、LPL和ADIPOQ基因的表达也受到显著抑制(P<0.05)。[结论]体外siRNA沉默NCOA2基因可抑制猪肌内前体脂肪的分化,这可能与PPARγ、FABP4、LPL、ADIPOQ等基因的低表达有关。

[Objectives] The study aims to investigate the role of nuclear receptor coactivator 2（NCOA2）in porcine intramuscular preadipocytes differentiation.[Methods] The expression pattern of NCOA2 during porcine intramuscular preadipocytes differentiation was detected by quantity real-time PCR（qRT-PCR）. NCOA2 was knockdowned by small interfering RNA （siRNA）fragments. The intramuscular preadipocytes differentiation ability was assessed via oil red O staining after NCOA2 down-regulation,and the expression levels of peroxisome proliferator activated receptor γ（PPARγ）,fatty acid binding protein 4（FABP4）,lipoprotein lipase （LPL） and adiponectin （ADIPOQ） were also detected by qRT-PCR.[Results] NCOA2 expression level was significantly up-regulated during preadipocytes differentiation at 4-day（P〈0.05）. The porcine intramuscular preadipocytes differentiation ability was significantly inhibited after NCOA2 down-regulation（P〈0.05）,and PPARγ,FABP4,LPL and ADIPOQ expression levels were also dramati-cally reduced（P〈0.05）.[Conclusions] The procine intramuscular preadipocytes differentiation can be inhibited by knockdown of NCOA2 in vitro,and this may be related to down-regulation of PPARγ,FABP4,LPL and ADIPOQ.