超高压协同温度处理对过敏原Bos d 6抗原性及二级结构的影响

Effect of combined ultra high pressure and temperature treatment on the allergenicity and secondary structure of allergen Bos d 6

[目的]本文旨在研究超高压协同温度处理对过敏原Bos d 6抗原性和二级结构的影响。[方法]采用间接竞争ELISA和圆二色光谱方法,研究在25、40和55℃条件下,100、200、300、400、500和600 MPa压力分别处理10 min对过敏原Bos d 6抗原性及二级结构的影响,并分析过敏原抗原性与其二级结构中α-螺旋的相关性。[结果]超高压处理能有效降低过敏原Bos d 6的抗原性及其二级结构中α-螺旋含量;随着处理温度的增加,过敏原抗原性和α-螺旋含量的降低幅度明显;超高压协同温度处理后过敏原Bos d 6抗原性与α-螺旋含量呈明显的正相关性。[结论]超高压协同温度处理技术可以通过改变过敏原Bos d 6蛋白质的空间构象来达到降低其抗原性的目的。

[Objectives] The effect of combined ultra high pressure and temperature treatment on the allergenicity and secondary structure of allergen Bos d 6 was investigated.[Methods] A study was carried out about the ultra high pressure（100,200,300,400,500 and 600 MPa）for 10 min at 25,40,and 55℃ on the allergenicity and secondary structure of allergen Bos d 6 using indirect competitive ELISA and circular dichroism spectroscopy. The correlation between the α-helix content and the allergenicity was analyzed by linear regression.[Results] The allergenicity and α-helix content in the secondary structure of allergen Bos d 6 could be effectively reduced by ultra high pressure,which decreased significantly with the temperature increasing. There was a significant positive correlation between the allergenicity and α-helix content of allergen Bos d 6 after the combined treatment of ultra high pressure and temperature treatment.[Conclusions] The combined ultra high pressure and temperature treatment could decrease the allergenicity of allergen Bos d 6 through changing their protein conformation.