功能性氧化石墨烯-β-环糊精/阿司匹林复合物的制备和质量评价及其抗凝血性

Preparation,quality evaluation and anticoagulant properties of functional graphene oxied-β-cyclodextrin/aspirin compound

目的:合成新型的功能性氧化石墨烯-β-环糊精/阿司匹林(GO-DEX-β-CD/ASA)复合物,并对其进行质量评价和抗凝血性研究。方法:利用电子扫描显微镜(SEM)和X-线衍射仪(XRD)对其形貌进行表征;对生成物的包封率、回收率以及精密度进行评价,以离心法测定GO-DEX-β-CD/ASA复合物的包封率;通过溶血试验和复钙试验评价其血液相容性。结果:GODEX-β-CD/ASA复合物的平均回收率(n=3)为(100.3±0.32)%,包封率(n=5)为(76.08±1.06)%,溶血率低于5%,符合生物医学材料的标准。结论:GO-DEX-β-CD/ASA复合物制备工艺可行,质量控制方法简便可靠,具有良好的血液相容性。

OBJECTIVE To synthesize new type GO-DEX-β-CD/ASA compound,evaluate qualities and anticoagulant properties.METHODS Microstructure of graphene oxide was characterized by scanning electron microscopy（SEM）and X-ray diffraction（XRD）.Recovery,precision and entrapment efficiency of GO-DEX-β-CD/ASA compound were studied,and GODEX-β-CD/ASA compound entrapment efficiency was determined by centrifugation.Blood compatibility of GO-DEX-β-CD/ASA compound was evaluated by hemolysis test and plasma recalcification time test.RESULTS Average recovery（n=3）of GO-DEX-β-CD/ASA compound was（100.3±0.32）%,with an entrapment efficiency（n=5）of（76.08±1.06）%.Hemolysis rate of GO-DEX-β-CD/ASA compound passed relevant international standard.CONCLUSION Preparation technique of GODEX-β-CD/ASA compound is feasible,quality control method is convenient and reliable,and blood compatibility is good.