原子荧光分光光度法检测人尿中锡

Detection of tin in human urine by atomic fluorescence spectrophotometry

目的建立尿中锡的原子荧光分光光度测定方法。方法取2.50 mL尿样加入1.50 mL浓硝酸后,用石墨消解仪消化,以体积分数为4.00%的盐酸溶液定容至10.00 mL,加入质量浓度为100 g/L的硫脲-抗坏血酸2.50 mL,以体积分数为4.00%的盐酸溶液定容至25.00 mL（相当于稀释尿样10倍）后,取1.00 mL样品采用原子荧光光度法进行测定。结果尿中锡在质量浓度为4.00～200.00μg/L范围内线性关系良好,相关系数为0.999 5;方法检出限为0.20μg/L,加标回收率为100.20%～100.84%,批内相对标准偏差（RSD）为0.11%～2.01%,批间RSD为1.37%～5.58%;样品在4℃条件下可保存7 d。结论本方法灵敏度高,准确性好,操作简便,适用于人尿中锡的日常监测。

Objective To establish a method for determination of urinary tin by atomic fluorescence spectrophotometry.Methods The graphite digestion instrument was used to digest 2. 50 m L urinary sample with 1. 50 m L concentrated nitric acid,hydrochloric acid（ volume fraction 4. 00%） was added to a total constant volume of 10. 00 m L. After 2. 50 m L of thiocarbamide-ascorbic acid（ mass concentration 100 g / L） was added,hydrochloric acid（ volume fraction 4. 00%） was added to a total constant volume of 25. 00 m L（ equivalent to urinary sample was diluted 10 times）,1. 00 m L of the sample was collected and detected by atomic fluorescence spectrophotometry. Results The good linear relationship was shown in the range of 4. 00-200. 00 μg / L with a correlation coefficient of 0. 999 5. The limit of detection was 0. 20 μg / L. The recovery rates ranged from 100. 20% to 100. 84%. The within-run relative standard deviation（ RSD） and between-run RSD were 0. 11%-2. 01% and 1. 37%-5. 58%,respectively. The samples can be stored for 7 days under the temperature of4 ℃. Conclusion This method has the advantages of high sensitivity,precision and convenient operation,which is suitable for the daily determination of urinary tin in human.