槲寄生化学成分的研究

Study on Chemical Constituents from Viscum coloratum(Komar.)Nakai

对槲寄生干燥茎叶的化学成分进行研究。采用反复硅胶、Sephadex LH-20及ODS等柱色谱技术进行分离纯化，根据理化性质及波谱分析鉴定化合物的结构。槲寄生干燥茎叶用95％乙醇提取浓缩后用水-氯仿萃取。其中水部位经D101型大孔树脂水：乙醇梯度（100：0～0：100）洗脱后得水和10％～95％乙醇6个部位，其中在95％乙醇中分离得到3个化合物，分别鉴定为鼠李秦素-3-O-β—D-[5″-（3-羟基-3-甲基戊二酸半酯）]-芹菜糖基-（1→2）-[6″-（3-羟基-3-甲基戊二酸半酯）]-O-β-D-葡萄糖苷（1）、鼠李秦素-3-O-β-D-[6″-（3-羟基-3-甲基戊二酸半酯）]-O—β—D-葡萄糖苷（2）和鼠李秦素-3-O—β—D-芹菜糖基-（1→2）-[6″-（3-羟基-3-甲基戊二酸半酯）]-O-β-D-葡萄糖苷（3）。其中化合物1为新的黄酮苷类化合物，命名为槲寄生新苷Ⅷ。

The chemical constituents from the dry stems and leaves of Viscum coloratum （Komar.）Nakai were isolated and purified by various chromatographic methods and their structures were elucidated on the basis of detailed spectroscopic analysis. The dry stems and leaves of Viscum coloratura （Komar.）Nakai was extracted by 95 % ethanol, followed by concentration and extraction by water and chloroform. Then the water part was eluted in D101- type macroporous resin by the gradient elution of water：ethanol （100 ： 0-0 ： 100） to obtain water and 10%-95 % ethanol six parts. Three compounds were isolated from the 95 % ethanol part and identified as rhamnazin-3-O-β-D-[5″- （3-hydroxy-3-methylglutaryl） ] -api- （1→2） - [6″- （3-hydroxy-3-methylglutaryl） ] -O-β-D-glucoside （1）, rhamnazin-3-O- β-D-[6″-（3-hydroxy-3-methylglutaryl）]-O-β-D-glucoside （2） and rhamnazin-3-O-β-D-api-（1→2）-[6″-（3-hydroxy-3- methylglutaryl） ] -O-1β-D-glucoside （3）. Compound l is a new flavonoid glycoside, named as viscumneoside Ⅷ.