楂曲平胃合剂中6个成分的HPLC法测定

Determination of Six Constituents in Zhaqu Pingwei Mixture by HPLC

建立了高效液相色谱法测定楂曲平胃合剂中的和厚朴酚、厚朴酚、牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素和金丝桃苷6个成分。采用Kromasil C18色谱柱，以25％乙腈甲醇溶液为流动相A，0．5％甲酸为流动相B，梯度洗脱；检测波长为294nm（和厚朴酚、厚朴酚）和340nm（牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素和金丝桃苷）。结果上述6成分分别在4．75～95.0、5.00～100.0、2．50～50.0、6．50～130.0、2．00～40.0和3．25～65．0μg/ml范围内线性关系良好，平均回收率分别为98．9％、97．8％、99-3％、98．5％、97．9％和97．2％，RSD分别为1．44％、1．08％、1.21％、0.98％、1．30％和1．52％。

An HPLC method was established for the determination of honokiol, magnolol, glucosylvitexin, vitexin-2-O-rhamnoside, vitexin and hyperoside in Zhaqu Pingwei mixture. A Kromasil C18 column was used, with the mobile phase A of 25 % acetonitrile methanol solution and the mobile phase B of 0.5 % formic acid by gradient elution, at the detection wavelengths of 294 nm （for the detection of honokiol and magnolol） and 340 nm （for the detection of glucosylvitexin, vitexin-2-O-rhamnoside, vitexin and hyperoside）. The linear ranges for the above six components were 4.75-95.0, 5.00-100.0, 2.50-50.0, 6.50-130.0, 2.00-40.0 and 3.25-65.0 μg/ml, respectively. Their average recoveries were 98.9%, 97.8%, 99.3%, 98.5%, 97.9% and 97.2%, with RSDs of 1.44%, 1.08%, 1.21%, 0.98%, 1.30% and 1.52 %, respectively.