G蛋白偶联受体87对奶牛乳腺上皮细胞泌乳的影响

Effects of GPR87 on lactation in dairy cow mammary epithelial cells

为研究G蛋白偶联受体87(GPR87)在氨基酸调控泌乳过程中的作用,在奶牛乳腺上皮细胞中分别添加浓度为0.6mmol/L蛋氨酸(Met)和1.2 mmol/L赖氨酸(Lys),检测细胞泌乳能力变化及细胞中GPR87的表达。结果表明,1添加Met和Lys均能使细胞泌乳能力和细胞中GPR87的表达显著增加;对GPR87进行过表达和抑制,检测细胞泌乳能力变化;GPR87过表达后,细胞泌乳能力显著增加,抑制后,细胞泌乳能力显著下降;2抑制GPR87同时添加Met或Lys,检测细胞中CSN2、m TOR、SREBP-1c、GLUT1的表达及细胞泌乳能力的变化;3与只添加氨基酸组比较,抑制GPR87同时添加氨基酸组,细胞中CSN2、m TOR、SREBP-1c、GLUT1的表达及细胞泌乳能力的增加程度显著下降。综合结果说明,GPR87能接受并传递氨基酸信号,正向调控奶牛乳腺上皮细胞的泌乳。

The aim of this study was to investigate the effect of G protein coupled receptors 87 （GPR87） on the process of amino acid-medi- ated regulation of lactation in dairy cow mammary epithelial cells （DCMECs）. The DCMECs were treated with 0.6 mmol/L methionine （Met） and 1.2 mmol/L lysine （Lys）, respectively, the lactation and GPR87 expression of these cells were detected, the result showed that lacta- tion and GPR87 expression of DCMECs were significant increased by Met or Lys stimulus; GPR87 was overexpressed and inhibited in DC- MECs, respectively, the lactation of these cells were detected, the result showed that lactation of DCMECs was significant increased by GPR87 overexpression and significant reduced by GPR87 inhibiton; Treated with GPR87 inhibition and Met or Lys stimulus, the lactation and the expression of CSN2, mTOR, SREBP-lc, GLUT1 of DCMECs were detected, the result showed that compared with only treated with Met or Lys stimulus group, the increase of lactation and the expression of CSN2, mTOR, SREBP-lc, GLUT1 of DCMECs treated with GPR87 inhibition and Met or Lys stimulus was significant reduced. Taken together, these results reveal that GPR87 as a regulation molecular could receive and pass the amino acid signal, and positive control the lactation of DCMECs.