微生物源性抗氧化剂对小鼠睡眠及抗氧化性能的影响

Effects of microbe-derived antioxidant on sleep and antioxidant ability in the rat

目的探究不同剂量微生物源性抗氧化剂对小鼠睡眠及其抗氧化性能的影响。方法 60只雄性昆明小鼠随机分为4组,低、中、高剂量组分别灌喂0.5 g/kg体重、1.0 g/kg体重和1.5 g/kg体重微生物源性抗氧化剂,对照组使用生理盐水进行灌胃,试验期30 d。在末次灌胃后,各组动物腹腔注射戊巴比妥钠,通过翻正反射实验观察小鼠睡眠状况,并在结束后,检测小鼠血清抗氧化性能。结果与低、高剂量组相比,中剂量组的微生物源性抗氧化剂可以显著延长戊巴比妥钠睡眠时间(P<0.05)。与对照组,低,高剂量组相比,中剂量组极显著提高GSH-Px活力(P<0.01)、显著提高SOD活力(P<0.05)。与对照组相比,中剂量组MDA和8-ISO-PGF2α含量有显著降低(P<0.05)。结论提示微生物源性抗氧化剂有促进小鼠的睡眠、提高机体抗氧化能力的作用,且在1.0g/kg体重剂量下效果最显著。

Objective To investigate the effect of different doses of microbe-derived antioxidant on sleep and antioxidant ability in mice. Methods Sixty male Kunming mice with similar body weight were randomly divided into 4groups. The control group received normal saline,and the experimental groups received microbe-derived antioxidant in a dose of 0. 5 g / kg bw,1. 0 g / kg bw or 1. 5 g / kg bw once per day,respectively. The experiment period was 30 days. At the end of experiment,the mice of each group were intraperitoneally injected sodium pentobarbital to induce sleep. The mice fall sleep was judged by righting reflex. After the test of sleep,blood was taken for detection of serum antioxidant ability.Results Compared with the low dose and high dose groups,the middle dose group showed a significantly prolonged sodium pentobarbital-induced sleeping time（ P 0. 05）. Compared with the control group,low and high dose groups,the middle dose group had highly significantly increased GSH-Px activity（ P 0. 01） and significantly increased content of SOD. Under these conditions,the middle dose group reduced both the contents of MDA and 8-ISO-PGF2α（ P 0. 05）compared with the control group. Conclusions Our results suggest that microbe-derived antioxidant exerts effect on sleep and antioxidant ability in rats. Supplement of 1. 0 g / kg bw / d shows the most significant effects.