先天性白内障小鼠主要脏器重量和血液生理生化指标的分析

Analysis of the main organs,hematological and biochemical indexes in congenital cataract mice

目的建立SPF级先天性白内障小鼠体重及主要脏器重量,血液生理生化等指标的背景资料。方法以28日龄及56日龄正常KM小鼠为对照组,以同日龄先天白内障小鼠为实验组,分别测定小鼠体重、主要脏器重量以及血液生理生化指标。结果与28日龄KM小鼠相比较,同日龄白内障小鼠各项指标均无统计学意义;与56日龄KM小鼠相比较:(1)小鼠体重、心、肝、脾、肺、肾、雄鼠睾丸重量差异均有统计学意义(P<0.05或P<0.01)。(2)血液学检查数据显示:雌鼠的平均血小板压积、血小板分布宽度以及雄鼠的白细胞、血小板、淋巴细胞比率差异有统计学意义(P<0.01)。(3)血液生化学检查数据显示碱性磷酸酶、尿酸、葡萄糖、总蛋白、雄鼠谷丙转氨酶、白蛋白、雌鼠的尿素、甘油三酯差异有统计学意义(P<0.05或P<0.01)。结论 56日龄白内障小鼠与同日龄KM小鼠相比,体重及主要脏器重量以及部分生理生化指标有一定的差异。研究结果可以为科研人员提供参考数据。

Objective To establish the baseline data of body weight,main organ weights,hematological and biochemical indexes in SPF congenital cataract mice. Methods Body weight,main organs weights,hematological and biochemical indexes of the congenital cataract mice were determined at 28 days and 56 days of age,respectively. Normal KM mice in the same age were taken as control. Results There were no statistically significant differences in all indexes of the mice at 28 days of age. Compared with the 56-day old normal KM mice：（ 1） Statistically significant differences were found in the body weight,and weights of the heart,liver,spleen,lung,kidney and testis（ P 0. 05 or P 0. 01;（ 2）Statistically significant differences were found in hematological indexes WBC,PLT,MPV,LYMP,PDW for female mice and MPV,PDW for male mice（ P 0. 01）;（ 3） Among the biochemical indexes,there were also statistically significantdifferences in UREA,ALP,TP,UA,TG,GLU for female and ALT,ALP,TP,ALB,UA,GLU for male mice.Conclusions There are statistical differences in the body weight,main organ weights,hematological indexes and biochemical indexes between the congenital cataract mice and normal KM mice at 56 days of age. These results may provide a useful reference for future research.