L-选择素在缺血性脑卒中患者外周血中的表达及其意义

L-selectin in peripheral blood of patients with ischemic cerebral stroke and its significance

目的探讨L-选择素在缺血性脑卒中患者中的表达及其意义。方法选择缺血性脑卒中患者(病例组)和正常健康体检者(对照组)各50例,以甘油醛-3-磷酸脱氢酶(GAPDH)为内参,运用半定量RTPCR技术检测研究对象外周血中L-选择素的表达水平。结果与对照组比较,病例组L-选择素水平明显升高(2.96±0.71 vs 1.22±0.46,P<0.01)。结论 L-选择素在缺血性脑卒中患者外周血中表达增加,提示L-选择素可能与缺血性脑卒中的发病有关。

Objective To explore the expression of L-selectin in peripheral blood of patients with ischemic cerebral stroke,and the relationship between L-selectin and the ischemic stroke.Methods The expression of L-selectin in peripheral blood of 50 patients with ischemic cerebral stroke,as case group,and 50 normal subjects,as control group,was detected using the semi-quantitative RT-PCR technology,and the expression of glyceraldehyde 3 phosphate dehydrogenase（GAPDH）was used as the internal control.Results The expression of L-selectin in patients with ischemic cerebral stroke was significantly higher than that in normal subjects（2.96±0.71 vs 1.22±0.46,P〈0.01）.Conclusion The expression of L-selectin was higher in patients with ischemic cerebral stroke.It is inferred that L-selectin was probably involved in the onset of ischemic cerebral stroke.