小鼠不同动情周期子宫内膜组织中Bcl-2、Bax和Caspase-9蛋白的表达

Expressions of Bcl-2,Bax,and Caspase-9 proteins in mouse endometrium tissue at different stages of estrous cycle

目的:探讨Bcl-2、Bax和Caspase-9在性成熟期小鼠动情周期不同阶段子宫内膜组织中的表达及定位。方法:选取32只性成熟期健康未孕雌性昆明小鼠,通过阴道脱落细胞检测法,根据阴道涂片细胞种类,确定各小鼠所属动情周期阶段,将小鼠分4组(n=8),分别为动情前期组、动情期组、动情后期组、动情间期组。免疫组化法检测Bcl-2、Bax和Caspase-9蛋白在子宫内膜组织中的表达。结果:Bcl-2在子宫内膜上皮组织中表现出与动情周期相关的规律性表达,动情间期表达高于其他各期(P<0.05);Bax在各动情周期均有表达,不同动情周期子宫内膜组织中Bax蛋白的表达差异无统计学意义(P=0.796);Caspase-9在动情期子宫内膜组织中主要表达于黏膜上皮细胞胞质、固有层子宫腺细胞胞质、基质细胞胞质,且动情前期表达低于其他各期(P<0.05)。结论:Bcl-2、Bax、Caspase-9在动情周期中共同调节子宫内膜细胞周期性的增殖与凋亡。

Aim：To explore the expressions of Bcl-2,Bax,and Caspase-9 in endometrium at different stages of estrous cycle in mature mice.Methods：A total of 32 sexual maturity healthy mice were randomly allocated into 4 groups（n=8）, and through the rats vaginal smear observation and types of the cells , the mouse′s estrous cycle stage was clearly deter-mined.Then immunohistochemistry was carried out to screen the protein of Bcl-2,Bax,and Caspase-9 in mice.Results：Bcl-2 was highly appeared in diestrus stage （P〈0.05）.Bax was also expressed in the estrous cycle but had no significant difference among different stages （P=0.796）.Besides, Caspase-9 in each estrus stage of mainly expressed in the mucosa epithelial cytoplasm , lamina propria uterus gland cell cytoplasm ,and stromal cell cytoplasm ,and it was lowly expressed in the beginning of the estrous cycle （P〈0.05）.Conclusion：Bcl-2,Bax, and Caspase-9 regulate the proliferation and apop-tosis of endometrial cell cycle during estrous cycle .