不同年龄IVF-ET患者卵丘颗粒细胞端粒长度与卵母细胞成熟度及妊娠结局的关系

Relationship of telomere length in cumulus cells with oocytes maturation and outcome of IVF-ET in patients with different ages

目的:探讨体外受精-胚胎移植(IVF-ET)卵丘颗粒细胞的端粒长度与卵母细胞成熟度及妊娠结局的关系。方法:收集81例行体外受精-胚胎移植患者的卵丘颗粒细胞,根据患者的年龄,分为低龄(<35岁)组42例和高龄(≥35岁)组39例。应用qRT-PCR测量卵丘颗粒细胞端粒长度。结果:卵丘颗粒细胞端粒长度与年龄呈负相关(r=-0.267,P=0.021),低、高龄组内成熟卵母细胞的卵丘颗粒细胞端粒长度明显比未成熟卵母细胞的长(P<0.05)。高龄组妊娠者端粒长度较未妊娠者长(P<0.05)。结论:在IVF-ET中,卵丘颗粒细胞的相对端粒长度随年龄增长逐渐缩短,可反映卵母细胞的成熟度;端粒长度缩短可能会影响妊娠结局。

Aim：To investigate the relationship of the relative telomere length in cumulus cells （CCs） with oocytes at different mature stages and the outcome of in vitro fertilization and embryo transfer （IVF-ET）.Methods：Oocyte-cumulus complex samples were collected from 81 patients undergoing IVF-ET and CCs were manually separated .A total of 42 women （〈35 years,the younger group） and 39 women（≥35 years, the older group） were collected.The oocyte maturation and the result of clinical pregnancy were recorded in different groups .DNA was extracted from CCs and assessed for telomere length by real-time quantitative PCR .Results： There were negative correlation between relative telomere length of CCs with the patients′age （r=-0.267, P=0.021）.The relative telomere length of CCs was higher in mature oocytes than immature oocytes in both groups （P〈0.05）.There was a significantly higher telomere length of CCs in the pregnant sub-group than in the non-pregnant subgroup among the older group （P〈0.05）.Conclusion：In IVF-ET, the relative telomere length of CCs gradually shortened with age .The telomere length of CCs could reflect the degree of oocyte maturation and may be associated with pregnancy outcome in IVF-ET.