利用甜菜发状根产生甜菜色素的研究进展

Research Progress of Producing Betalains by Red Beet Hairy Root

发根农杆菌侵染植物后其Ri质粒上的一段DNA（T—DNA）可以在植物细胞基因组中进行整合并表达从而产生发根。相比于正常植株T—DNA的整合会使发根合成更多的次级代谢产物。此外发状根会产生更多的次生代谢产物并释放到培养基中从而为收集次级代谢产物带来了便捷。该发现为提高甜菜发状根中甜菜红素的产量提供了理论基础。此外，本文探讨了T—DNA的插入量及再次转化对甜菜发状根甜菜红素产量的影响并对发状根的生长影响因素及发状根的应用前景进行了介绍。

After Agrobacterium rhizogenes infested plants, some DNA （ T - DNA） of Ri plasmid can be carried out in the plant cell genome to have a integration and then express, resulting in the hairy root. Compared to nor- mal plants, T- DNA integration will make hair roots producing more secondary metabolites. In addition, more secondary metabolites will released into the medium, so as to it brings convenience for collecting secondary me- tabolites . This found provides a theoretical basis for increasing betalaine production in red beet hairy roots . What is more, this paper discusses the influence of the amount of T - DNA insertion and retransformed on beta- lainethe production, and introduces the growth factors and prospects of the hairy root.