小鼠异位肢体复合组织移植模型的建立

Establishment of a mouse model of limb heterotopic composite tissue allograft transplantation

目的建立稳定的小鼠异位肢体复合组织移植模型。方法选用25对C57BL/6小鼠行同品系异位肢体复合组织移植手术。取供体小鼠后肢,于腹股沟韧带下缘起沿股动脉、股静脉向上游离,电凝切断除股静脉、股动脉外所有分枝血管至髂外动脉、髂外静脉,断髂外动脉、髂外静脉后,横断股骨、股部肌肉。将供体髂外动脉、髂外静脉分别与受体小鼠右侧颈总动脉、颈外静脉行端侧吻合。连续缝合供体皮缘于受体皮肤切缘固定肢体。于供肢胫骨远端关节处切断,结扎残端。结果手术成功率76%,总手术时间(87.1±10.1)min,供体获取时间(23.8±4.9)min,血管吻合时间(55.3±9.6)min。结论小鼠异位肢体复合组织移植成功率较高,稳定可靠,是一种新的研究移植免疫的理想的动物模型。

Objective To establish a stable mouse model of limb heterotopic composite tissue allograft transplantation. Methods 25 pairs of C57 BL /6 mice were used for this limb heterotopic allograft transplantation experiment. The hind legs were taken from the donor mice. A transverse incision was made at the level of inguinal ligament to expose and mobilize the femoral vessels up to the external iliac vessels. All branches to the external iliac artery and vein were cut off by electrocoagulation. To cut off the external iliac artery and external iliac vein,and to transect the femur,thigh muscles and skin. The donor external iliac artery-external iliac vein anastomosis was performed in end-to-side fashion with recipient mice＇s right common carotid artery and jugular vein,respectively. Continuously suture the donor skin to the recipient skin incisal margin to fix the limb. Cut off the donor limb＇s distal tibial joint,and ligate the stump. Results The success rate of the heterotopic limb transplantation operation was 76%. The total operation time was 87. 1 ± 10. 1 minutes,and the vessel anastomosis time was 55. 3 ± 9. 6 minutes. Conclusions This mouse model of limb heterotopic limbcomposite tissue allograft transplantation shows a high success rate of the surgical operation,and is stable and reliable. It is a new ideal model for studies on transplantation immunology.