抗纤灵方抑制肾络病慢性肾衰竭大鼠TGF-β/P13K/Akt信号旁路的实验研究

Experimental Research on “Kang Xian Ling Decoction” to Inhibit TGF-β/P13K/Akt Signal Passage of Rats with Chronic Renal Failure

目的:采用5/6肾切除方法建立慢性肾衰竭大鼠模型,观察抗纤灵对5/6肾切除大鼠肾组织中TGF-β/PI3k/Akt信号通路的影响。方法:将SD大鼠分为正常组、模型组(生理盐水)、治疗组(抗纤灵)各12只,除正常组外均采用5/6肾切除方法建立慢性肾衰竭大鼠模型,疗程2个月。应用生化、放射免疫法、western blot等技术,检测肾功能、24 h尿蛋白定量、肾脏病理,检测TGF-β/P13K/Akt信号通路蛋白表达。结果:实验结束时,与模型组相比较,抗纤灵组的血肌酐数值显著下降(P<0.01)、蛋白尿下降差异有统计学意义(P<0.05);病理MASSON染色:抗纤灵组肾小球肥大减弱,毛细血管基底膜增厚减轻,系膜基质明显减少,胶原纤维成分低于模型组;western blot检测,与模型组相比,抗纤灵组的p-Akt灰度比值显著下降。结论:抗纤灵方可能通过抑制TGF-β/PI3K/Akt信号通路,从而有效地降低5/6肾切除大鼠的血肌酐,减轻肾组织纤维化、改善肾功能、降低蛋白尿。

Objective： Replicate 5 /6 nephrectomy CRF rat＇s models to observe the effects on TGF- β / P13 K / Akt signal passage of experimental rats with ＂Kang Xian Ling Decoction＂. Methods： Divide 36 rats into three groups（ normal group,model group with physiological saline,treatment group with the drug）; then replicate 5 /6 nephrectomy CRF rat＇s models except normal group for 2 months; apply medical technology such as biochemical mensuration of blood and organization,Radioimmunoassay and Western blot to detect renal function and 24 h UPro,wrenal pathology and TGF- β / P13 K / Akt signal passage. Results： After experiment,compared with model group,serum creatinineresults of mode group dramatically reduced（ P 0. 01）,serum creatinine of treatment group increase obviously（ P 0. 05）,Pathology observation MASSON; compared with model group,Hypertrophy on glomerulus and basilar membrane on capillary vessel of treatment group has weakened. There has been an obvious decrease in mesentery stroma;collagenous fibre elements is lower than model group; During western blot examination,compared with model group,gray value of p-Akt in treatment group decline obviously. Conclusion： Chinese medicine ＂Kang Xian Ling Decoction＂can significantly improve kidney function of 5 /6 nephrectomy CRF rats through reducing albuminuria and serum creatinine to protect renal function with inhibiting TGF- β / P13 K / Akt signal passage.