Nell-1基因启动子区甲基化在胃癌中的研究

Research on Nell-1 Promoter Methylation Patterns in Patients with Gastric Cancer

目的:探讨Nell-1基因启动子区不同甲基化位点与胃癌患者术后病理分期及淋巴结转移度之间相关性。方法:收集2012年4月至2012年9月在哈尔滨医科大学附属第四临床医院肿瘤外科25例接受胃癌根治术的病人资料,运用配对t检验及线性回归统计方法,分析Nell-1基因启动子区不同甲基化位点与胃癌患者临床病理资料之间的相关因素。结果:Nell-1基因启动子区不同甲基化位点的胃癌组织组和胃正常组织组甲基化率之间的差别有统计学意义(第1号位点及第38号位点),简单线性回归结果分析显示在第1号位点随着患者术后病理分期逐渐降低,胃癌组织组和胃正常组织组甲基化率之间的差值会逐渐增大(负相关);而在第38号位点随着淋巴结转移度的逐渐增大,胃癌组织组和胃正常组织组甲基化率之间的差值会逐渐增大(正相关)。结论:胃癌的发生受诸多因素的影响,不同甲基化位点其甲基化率不同,其与胃癌患者临床病理资料之间存在一定相关性。

Objective： To investigate the relationship between Nell-1 promoter methylation patterns and pathological data of gastric cancer patients. Methods： The clinical data of 25 cases of gastric cancer surgery performed from April to September 2012 in Department of Surgical Oncology, The Fourth Hospital of Harbin Medical University was included into the study. Using paired t test and linear regression analysis, the correlation factors of Nell-1 gene promoter methylation patterns and clinical pathological data of gastric cancer patients were analyzed. Results： The difference of methylation rates between gastric cancer tissues and normal gastric tissues in different methylation sites of Nell-1 gene promoter region was statistically significant （1 and 38）. Results of simple linear regression analysis showed that the difference of methylation rates between gastric cancer tissues and normal gastric tissues was gradually increased when the pathological stage of the patients gradually decreased（Negative Correlation） in the first site. The difference of methylation rates between gastric cancer tissues and normal gastric tissues was gradually increased when the degree of lymph node metastasis gradually increased （Positive Correlation） in the thirty-eight site. Conclusions： The occurrence of gastric cancer is influenced by many factors, and different methylation sites have different methylation rates. There is a correlation between the clinical pathological data of gastric cancer patients and the difference ofmethylation rates in different methylation sites.