急性脑梗死患者血清中miR-21-5p与炎性因子的相关性分析

The correlation analysis of serum miR-21-5p and inflammatory factor in acute cerebral infarction patients

目的探讨急性脑梗死患者外周血miR-21-5p与炎性因子白细胞介素6（interleukin-6,IL-6）、IL-8、C反应蛋白（C-reactionprotein,CRP）的相关性。方法收集首次发病的急性脑梗死患者64例,病程24~48h,同期选择健康体检者60例为对照组。实时荧光定量PCR方法检测2组血清中miR-21-5p的表达量,ELISA检测血清IL-6、IL-8及CRP的水平,并与miR-21-5p进行相关性分析。分别在入院时及出院后检测急性脑梗死患者的美国国立卫生研究院卒中量表（NIH Stroke Scale,NIHSS）评分和日常生活活动能力（Activities of Daily Living,ADL）评分。结果脑梗死组血清miR-21-5p、IL-6、IL-8、CRP水平均高于对照组,且miR-21-5p与IL-6、IL-8、CRP表达水平均呈正相关。出院后miR-21-5p相对低表达组NIHSS评分低于miR-21-5p相对高表达组,ADL评分高于miR-21-5p相对高表达组。结论急性脑梗死患者血清中miR-21-5p表达上调与血清炎性因子IL-6、IL-8以及CRP的水平呈正相关。miR-21-5p有可能作为预测急性脑梗死预后的一种潜在的生物学标志。

Objective To investigate the correlation between serum miR-21-5p and inflammatory cytokines interleukin-6（IL-6）,IL-8and C-reactionprotein（CRP）in patients with acute cerebral infarction（ACI）.Methods Blood samples were collected from 64 inpatients with acute cerebral infarction（duration 24-48h）and from 60 healthy physical examinees with matching age and gender as control group.Real-time quantitative PCR was performed to examine the expression profiles of serum miR-21-5p in ischemic stroke patients and in control subjects.The ELISA method was used to detect the level of serum IL-6,IL-8and CRP from two groups.Correlation analysis was made between miR-21-5p and inflammatory factors.NIH Stroke Scale（NIHSS）scores and Activities of Daily Living（ADL）scores analysis were used to analyze the clinical prognosis of cerebral infarction patients within and after admission.Results The levels of serum miR-21-5p,IL-6,IL-8,CRP in the cerebral infarction group were higher than those in the control group.And miR-21-5p were positively correlated with the expression levels of IL-6,IL-8and CRP in ACI patient.After discharge,the NIHSS score of miR-21-5p in relatively low expression group was lower than that in relatively high expression group,while the ADL score of miR-21-5p in relatively low expression group was higher than that of miR-21-5p in relatively high expression group.Conclusion The up-regulated miR-21-5p expression in serum of patients with acute cerebral infarction was positively correlated with the level of serum inflammatory factors IL-8,IL-6and CRP.MiR-21-5p may serve as a potential biomarker for predicting the prognosis of patients with acute cerebral infarction.