分子印迹技术应用:真菌发酵液中紫杉醇的分离富集

Separation and Enrichment of the Paclitaxel from Fungal Fermentation Liquid by Molecular Imprinting Technology

紫杉醇是一种二萜类化合物,主要具有抗癌活性.以紫杉醇为模板分子合成分子印迹聚合物,并从吸附动力学和吸附等温曲线两方面对聚合物的吸附性能进行评价,结果表明此印迹聚合物具有一定的吸附能力.将合成的印迹聚合物作为固相萃取材料从真菌发酵液中分离富集紫杉醇,粗提物经过分子印迹柱萃取后,能够专一性的富集紫杉醇.通过建立快速、简便的富集紫杉醇的分离体系,将为具有显著抗癌作用的紫杉醇的临床应用奠定基础,具有很好的应用前景.

Paclitaxel, a naturally occurring diterpenoid compound, has been reported to have a mainly anticancer activity. The MIP was synthesized by paclitaxel as the template, and its adsorption behavior investigated in detail, including adsorption kinetics and equations of the molecularly imprinted polymers. The MIP exhibited some selectivity and affinity for paclitaxel compared with its structure analogues. A method was developed to separation and enrichment paclitaxel from fungal fermentation liquid using a molecularly imprinted polymer as the sorbent material. After per-concentrate by the MIP, paclitaxel can be separated and adsorpted. The approach provided a fast and simple method for obtaining paclitaxel from fungal fermentation liquid, which can provide a significant foundation for the clinical application of anticancer drug and have a good application prospect.