摘要
目的：建立高效液相色谱法测定半夏杏仁汤中苦杏仁苷的含量。方法：采用Thermo-C18柱（4.6mm×250mm,5μm）;流动相：甲醇-乙腈-水（10∶10∶80）;流速：1.0mL·min^-1;波长：210nm;柱温：25℃,进样量20μL。结果：苦杏仁苷在0.150 4-2.256 0μg内具有良好线性关系（r=0.999 9）,平均回收率为98.06%,RSD为1.12%（n=6）。结论：该方法简便、重现性好,可用于半夏杏仁汤及其制剂的质量控制。
Objective：To establish a determination of amygdalin content in pinellia almond soup by HPLC.Methods：The ThermoC18column（4.6mm×250mm,5μm）;methanol-acetonitrile-water（10∶10∶80）as mobile phase;flow rate of 1.0mL·min^-1;detection wavelength of 210nm;column temperature was 25 ℃,sample amount to 20μL.Results：Amygdalin within 0.150 4-2.256 0μg has a good linear relationship（r=0.999 9）,the average recovery was 98.06%,RSD was 1.12%（n=6）.Conclusion：This method is simple and good reproducibility,and can be used in pinellia almond soup and preparation of quality control.
出处
《亚太传统医药》
2016年第13期31-32,共2页
Asia-Pacific Traditional Medicine
