青海高原地区胎儿生长受限患者胎盘组织人类白细胞相关抗原G表达的研究

A Study on HLA-G in Maternal Placentas with Intrauterine Growth Retardation at Plateau Area

目的：探讨青海高原地区胎儿生长受限（FGR）患者人类白细胞相关抗原G（HLA-G）mRNA及蛋白的表达及临床意义。方法：收集2015年1~12月在青海大学附属医院产科分娩的20例FGR患者（FGR组）和20例正常妊娠妇女（正常妊娠组）的胎盘组织,采用实时荧光定量PCR及Western blot分析检测HLA-G mRNA及蛋白在两组胎盘组织中的表达差异。结果：FGR组胎盘组织中HLA-G mRNA表达水平（0.449±0.116）,明显低于正常妊娠组（0.963±0.226）,差异有统计学意义（P=0.000）。FGR组胎盘组织HLA-G蛋白表达灰度值（0.375±0.101）,亦明显低于正常妊娠组（0.797±0.177）,差异有统计学意义（P=0.002）。结论：青海高原地区FGR患者胎盘组织HLA-G mRNA及蛋白表达水平均明显降低,HLA-G的表达量降低在FGR的发生发展过程中可能起着重要的作用。

Objective： To investigate the expression and the clinical significance of HLA-G at the mRNA and the protein levels in fetal growth restriction（ FGR） patients at the plateau area of Qinghai.Methods： The placental tissue sample of 20 normal pregnant women（ normal pregnancy group）,20 patients with FGR（ FGR group） were collected.Using real time polymerase chain reaction and Western blot analysis,we detected HLA-G mRNA and protein levels in placental tissues.Results： HLA-G mRNA in FGR group（ 0. 449 ± 0. 116） was significantly lower than that in control group（ 0. 963±0. 226,P = 0. 000）.HLA-G protein in FGR group（ 0. 375±0. 101） was significantly decreased as compared with that in normal pregnant women（ 0. 797±0. 177,P = 0. 002）.Conclusions： In Qinghai plateau,the HLA-Gm RNA and protein levels decrease in the placenta tissue of FGR patients.The decreasing of HLA-G mRNA and protein expression may play an important role in the development and occurrence of FGR.