藏药独一味及其伪品HPLC特征图谱和4种成分含量测定

HPLC specific chromatogram of Lamiophlomis Herba and its counterfeit and determination of four effective components

采用高效液相色谱法建立独一味及其伪品的特征图谱,并同时测定4种主要成分的含量。以绿原酸、连翘酯苷B、毛蕊花糖苷和木犀草苷为对照品,采用Waters XSelect C_(18)色谱柱(4.6 mm×250 mm,5μm),乙腈-0.5%磷酸水溶液(18∶82)为流动相,检测波长332 nm,流速1.0 m L·min^(-1),柱温30℃进行试验,采用CHEMPATTERN化学计量学软件对结果进行处理分析。不同批次的独一味特征图谱相似度高,和伪品比较相似度均小于0.65,采用聚类分析和主成分分析均能将伪品区分。所建立的独一味特征图谱专属性强,可结合4种主要有效成分含量测定全面控制独一味的质量,为规范独一味药材的使用提供科学依据。

This study is to establish the HPLC specific chromatogram and determine four main effective components of Lamiophlomis Herba and its counterfeit. Chlorogenic acid,forsythoside B,acteoside and luteoloside were reference substance. HPLC analysis was performed on a Waters XSelect C_（18）column（ 4. 6 mm × 250 mm,5 μm）. The mobile phase was acetonitrile-0. 5% phosphoric acid solution（ 18∶ 82） with isocratic elution. The flow rate was 1. 0 m L·min^（-1）,the detection wavelength was 332 nm and the column temperature was 30 ℃. Chemometrics software Chempattern was employed to analyze the research data. HPLC specific chromatogram of Lamiophlomis Herba from different samples were of high similarity,but the similarity of the HPLC specific chromatogram of its counterfeit were less than 0. 65. Both of cluster and principal component analysis can distinguish certified products and adulterants. The HPLC specific chromatogram and contents of four effective components can be used for the quality control of Lamiophlomis Herba and its preparations. It provided scientific basis to standardize the use of the crude drug.