虎眼万年青提取物对肝纤维化的治疗作用研究

Research ontherapeutics effect of extract of Ornithogalum caudatum on liver fibrosis

采用四氯化碳(CCl4)制备小鼠肝纤维化模型,检测AST,ALT,γ-GT,MDA,GSH-px,SOD等血清指标,ELISA方法检测PCⅢ,IV-C,LN,HA等血清标记物;肝组织采用HE和Masson胶原染色,光镜下观察病理学改变;Western blot检测Ⅲ型胶原、TGF-β,α-SMA,E-cadherin蛋白表达,观察虎眼万年青提取物对CCl_4诱导的小鼠肝纤维化治疗作用,并探讨作用机制。结果表明,虎眼万年青提取物组(50,150,500 mg·kg^(-1))均可明显降低血清AST,ALT,γ-GT,MDA水平,升高GSH-px,SOD水平;降低肝纤维化血清标志物PCⅢ,IV-C,LN,HA的表达;明显改善CCl_4所致大鼠肝纤维化的病理学改变,证明虎眼万年青提取物对肝纤维化具有治疗作用。其机制是可能与抑制细胞外基质沉积,抑制肝星形细胞活化以及抑制上皮间质转化,进而抑制肝脏纤维化有关。

[Abstracr] Rat models of liver fibrosis were made by carbon tetrachloride,and the serum levels of AST,ALT,γ-GT,MDA,GSHpx,SOD were detected,serum markers of PCⅢ,IV-C,LN,HA were detected by ELISA method. HE and Masson staining were conducted in hepatic tissues to observe pathological variations. Collagen Ⅲ,TGF-β,α-SMA,E-cadherin were detected by Western blot.The curative effect of the extract of Ornithogalum caudatum on rat liver fibrosis induced by CCl_4 was observed and the mechanism was discussed. The experiment results showed that the extract of O. caudatum（ 50,150,500 mg·kg^（-1）） obviously decreased the serum levels of AST,ALT,γ-GT,MDA,increased the serum levels of GSH-px,SOD,decreased the expression of serum markers of PCⅢ,IV-C,LN,HA,and improved the liver pathological variations of fibrotic rats. The experiment proved that the extract of O. caudatum could treat the liver fibrogenesis induced by CCl_4 in rats. The positive medicine may inhibit accumulation of extracellular and activate hepatic stellate cell and epithelial-mesenchymal transition.