厨师人群基因组DNA总甲基化水平及影响因素分析

Genomic DNA total methylation level and its influencing factors in cooks

目的探讨烹饪油烟(cooking oil fume,COF)对机体基因组DNA总甲基化水平的影响。方法选取哈尔滨市内宾馆、酒楼等餐饮业从业人员,以120名中餐厨师作为COF暴露人群,以120名从事后勤、市场采购、客房、前台等人员作为对照人群,进行流行病学问卷调查,并采集血液进行基因组DNA总甲基化水平检测。结果 COF暴露组和对照组人群的年龄、受教育程度、吸烟情况、二手烟暴露、工龄等因素差异无统计学意义(P>0.05)。两组人群DNA均有不同程度甲基化,但暴露组人群DNA总甲基化水平显著高于对照组(P<0.05)。多元回归分析表明COF暴露量是影响厨师DNA总甲基化水平的危险因素,COF暴露量越大,DNA总甲基化水平越高。结论在本研究条件下,长期暴露于COF的厨师人群DNA总甲基化水平显著高于一般人群,DNA总甲基化水平与COF暴露量成正相关,DNA总甲基化水平可作为COF暴露的候选效应生物学标志物,应用于厨师人群肿瘤的早期筛查。

Objective To explore the effect of cooking oil fumes （COF） on total genomic DNA methylation level. Methods 120 Chinese food cooks from hotels and restaurants in Harbin city were selected as COF exposure group; 120 general staffs such as support crew, buyers, room attendants, receptionists, etc. were selected as controls. Epidemiologieal questionnaire was used and the blood samples of all the study subjects were collected for detection of genomic DNA total methylation level. Results There was no significant difference between COF group and control group in age, education level, smoking or secondhand smoke exposure, working years, etc. （P〈0. 05 ） , but the DNA total methylation level was significantly higher in COF group than that in control group （P〈0. 05）. Multiple regression analysis showed that COF exposure level was the risk factor of DNA total methylation level in cooks, heavier COF exposure may cause higher DNA total methylation level. Conclusion The results suggested that DNA total methylation level of cooks who exposed to COF is significantly higher than that of general population, the level is closely related to COF exposure amount. Therefore, DNA total methylation level may be considered as a candidate biomarkers of COF exposure.