摘要
为了解云南省疫苗衍生脊髓灰质炎病毒(Vaccine-derived Poliovirus,VDPV)的基因组特征,对2010年及2012年监测到的4株VDPV进行全基因组序列测定。结果显示,2株Ⅱ型VDPV的基因组全长均为7439nt,与Sabin Ⅱ疫苗株全基因组核苷酸和氨基酸的序列同源性分别为95.4%和97.7%;2株I型VDPV基因组全长均为7441nt,与Sabin I疫苗株全基因组核苷酸和氨基酸序列的同源性分别为93.9%和97.9%。减毒位点分析发现II型和I型VDPV毒株分别有两个(nt 481和nt 2909)和三个减毒位点(nt 480、nt 2795和nt 6203)发生了回复突变。VP1序列分析显示II型和I型VDPV毒株与相应Sabin株的变异分别为1%和2.3%,重组分析显示II型和I型VDPV的基因组结构分别为S2/S3和S1/S2/S1/S3,后者的重组次数高达3次,显示了重组的普遍性和复杂性,也表明了病毒在人体内复制和传播的持久性与重组的多样性成正相关。因此,从分子水平分析VDPV的特性,可掌握病毒的变异动态,为制定科学可行的VDPV控制策略提供理论依据。
To explore the genomic characterization of 4vaccine-derived poliovirus（VDPV）strains isolated from 2acute flaccid paralysis（AFP）cases in Yunnan Province in 2010 and 2012,respectively,the complete genome sequences of the 4strains were determined.Sequence analysis revealed that the complete genome length of the type Ⅱ and type Ⅰ VDPV was 7439 nt and 7441 nt,respectively.Nucleotide and amino acid sequence similarities of type II VDPV were 95.4% and 97.7%,respectively,and type I VDPV were93.9% and 97.9%,respectively as compared with those of Sabin strains.Nucleotide substitutions were found at two important attenuation sites（nt 481 and nt 2909）in type Ⅱ VDPV,and three important attenuation sites（nt480,nt2795 and nt6203）in typeⅠ VDPV.Type 2and type 1VDPV strains had 1.0% and2.3% divergence with Sabin strains,respectively.Similarity plot analysis showed multiple recombination events in the genome of the 4strains,which showed that the recombination was common and complex.Analysis of the characteristics of VDPVs on molecular level could provide valuable information on evolutionary dynamics and lay foundation for developing scientific and feasible strategy to control VDPV.
出处
《病毒学报》
CAS
CSCD
北大核心
2016年第4期459-464,共6页
Chinese Journal of Virology
基金
国家自然科学基金(81160198)
题目:云南省肠道病毒分子生物学特征及监测研究
