长链非编码RNAC6orf176两个转录本在非小细胞肺癌中的表达及意义

Expression of two transcript variants of long noncoding RNA C6orf176 in non-small cell lung cancer and its clinical significance

目的：探讨长链非编码RNA C6orf176两个转录本C6orf176-TV1和C6orf176-TV2在非小细胞肺癌（non-small cell lung cancer,NSCLC）中的表达及临床意义。方法：采用实时荧光定量PCR技术检测57例NSCLC组织及配对癌旁组织中C6orf176-TV1与C6orf176-TV2的表达水平,并分析其与临床病理特征的关系。结果：NSCLC组织中C6orf176-TV1表达下调42例,表达上调15例,其表达与肿瘤分化程度有关（P〈0.05）,与性别、年龄、吸烟史、病理类型、TNM分期均无关。C6orf176-TV1表达诊断癌组织与癌旁组织的受试者工作特征（receiver operator characteristic,ROC）曲线下面积（area under curve,AUC）为0.708（95%CI：0.615~0.802）,灵敏度和特异度分别为51%和88%。NSCLC组织中C6orf176-TV2表达下调39例,表达上调的18例,其表达与肿瘤分化程度有关（P〈0.05）,与性别、年龄、吸烟史、病理类型、TNM分期均无关。C6orf176-TV2表达诊断癌组织与配对癌旁组织的ROC的AUC为0.64（95%CI：0.531~0.749）,灵敏度和特异度分别为49%和75%。57例标本中C6orf176-TV1与C6orf176-TV2同时上调或下调的标本有53对,其相关系数为0.99。结论：C6orf176-TV1与C6orf176-TV2在NSCLC组织中均表达下调,其与肺癌肿瘤细胞分化程度相关,可作为NSCLC的诊断指标。

Objective To explore two transcript variants expression of long noncoding RNA C6orf176 in non- small cell lung cancer （NSCLC） and the clinical pathological significance. Methods： The expressions of transcript variant 1 （TV1） and transcript variant 2 （TV2） of long noncoding RNA C6orf176 in 57 NSCLC and adjacent cancerous tissues were examined by qPCR with actin as internal control. Results： Based on the results of qPCR, for C6orf176-TV1, 42 cases were down-regulated and 15 cases were up-regulated. The C6orf176-TV1 level was correlated to the grade of differentiation （P〈0.05） but it was not correlated with gender, age, smoking history, tumor type and TNM stage. The expression of C6orf176-TVI had a potential value in diagnosis of NSCLC by receiver operator characteristic （ROC） curve. Area under curve （AUC） of ROC curve was 0.708 （95% CI 0.615 to 0. 802）. The sensitivity and specificity were 51% and 88%, respectively. For C6orf176-TV2, 39 cases were down-regulated and 18 cases were up-regulated, The C6orf176-TV2 level was correlated with the grade of differentiation （P〈0.05） but it was not correlated with gender, age, tumor size, smoking history and TNM stage. C6orf176-TV2 level had value in diagnosis of NSCLC by ROC curve. AUC of ROC curve was 0.64 （95% CI 0.531 to 0.749）. The sensitivity and specificity were 49%and 75%, respectively. Of the 57 specimens, 53 cases were simultaneous up or down-regulation of C6orf176-TV1 and C6orf176-TV2. The correlation coefficient was 0.99. Conclusion： The expression of Cborf176-TV1 or and it is correlated with the grade of differentiation. patients. C6orf176-TV2 is down-regulated in NSCLC It may act as a diagnosis indicator for NSCLC