肝细胞肝癌组织表皮生长因子受体表达的临床病理学特征分析

Clinicopathologic characteristics of epidermal growth factor receptor expression in hepatocellular carcinoma

目的 :检测肝细胞肝癌(hepatocellular carcinoma,HCC)患者癌旁肝组织中表皮生长因子受体(epidermal growth factor receptor,EGFR)的表达情况,并分析其临床病理意义。方法 :采用免疫组织化学法检测EGFR在158例癌旁组织及HCC组织中的表达,结合临床病理资料统计分析。结果:免疫组织化学染色显示EGFR蛋白阳性定位于HCC细胞和癌旁肝细胞的细胞膜及细胞浆;EGFR在癌旁组织和HCC组织中阳性表达率分别为65.8%(104/158)、58.2%(92/158),癌旁组织中EGFR蛋白表达高于HCC组织,但差异无统计学意义(P>0.05);EGFR在癌旁肝组织中的表达与有无肝硬化情况(r=0.175 3,P=0.027 6)及术后复发情况(r=0.212 5,P=0.015 6)相关,而与HCC患者年龄、性别、HBs Ag感染、血清甲胎蛋白水平、肿瘤直径、肿瘤个数、组织学分级及血管侵犯均无关。结论:EGFR在癌旁肝组织中高表达,并且与肝硬化情况及术后复发有关,提示EGFR可能参与HCC的发生发展过程,检测癌旁肝组织中EGFR表达有助于预测HCC复发。

Objective ：To investigate the expression of epidermal growth factor receptor（EGFR） in paracancerous liver tissues of hepatocellular carcinoma（HCC） patients and to analyze its correlation with clinicopathological features. Methods： 158 samples of HCC, corresponding adjacent liver tissues were collected. Immunohistochemical staining was performed to detect the EGFR expression in corresponding adjacent liver tissues and HCC tissues. The correlation between EGFR expression and clinicopathological features of HCC was analyzed. Results： Immunohistochemical staining showed EGFR expression demonstrate cellular membrane and cytoplasmic reactivity in paracancerous liver cells and HCC cells. The positive rate of EGFR expression in corresponding adjacent liver tissues and HCC tissues were 65.8%（104/158） and 58.2% （92/158）. The EGFR expression was correlated with liver cirrhosis （r=0.175 3, P=0.027 6） and relapse （1-=0.212 5, P=0.015 6）, but not correlated with age, gender, the hepatitis B virus, the level of serum AFP, diameter of tumor, tumor number, differentiation degree of HCC and vascular invasion. Conclusion： High EGFR expression may take part in the HCC genesis and development process. Detecting the expression of EGFR may be helpful in prediction of early recurrence of HCC patients.