滋肾健脾化瘀片的质量标准研究

Specification for Zishenjianpihuayu Tablets

目的建立滋肾健脾化瘀片的质量控制方法。方法采用薄层色谱法鉴别滋肾健脾化瘀片的酒萸肉、三七、大黄;采用高效液相色谱法测定葛根素的含量。结果薄层色谱法斑点清晰、分离度好,且各阴性对照无干扰;葛根素在进样量为0.05~0.50μg范围内线性关系良好（r=0.999 9）,平均加样回收率为100.43%,RSD为1.39%（n=6）。结论该方法专属性强、灵敏度高、重复性好,可用于滋肾健脾化瘀片的质量控制方法。

OBJECTIVE To establish the specification for Zishenjianpihuayu tablets. METHODS Thin layer chromatography（ TLC） was used to identify Cornus officinalis Sieb. et Zucc,Panax notoginseng（ Burk.） F. H. Chen and Rheum palmatum L. in Zishenjianpihuayu tablets. The content of puerarin was determined by high performance liquid chromatography（ HPLC）. RESULTS The TLC spots were clear with good resolution and free of interference of negative samples.The puerarin showed good linear relationship in the range of 0.05-0. 50 μg（ r = 0. 999 9）. The average recovery was 100. 43%,RSD = 1. 39%（ n = 6）. CONCLUSION The method is simple,specific and durable.