界面亲疏水性对蛋白质聚集、结晶的影响

Influence of Surface Hydrophilicity and Hydrophobicity to the Aggregation and Crystallization of Protein

用硅烷偶联剂与多孔二氧化硅小球反应制备了6种不同化学基团修饰的多孔硅球,研究该界面材料的亲疏水性对蛋白质聚集和结晶行为的影响。通过动态光散射(DLS)和原子力显微镜(AFM)对溶菌酶溶液中聚集体粒径的表征发现:材料的多孔结构能够促进无序多聚体的解聚,防止其阻碍晶体生长;此外,若多孔硅球的界面疏水,则能加快这一过程,使溶液迅速达到只有寡聚体的单分散状态,从而提高结晶速率,亲水界面则相反。扫描电镜(SEM)观察材料界面的晶体形貌进一步表明:具有疏水界面的多孔硅球能更快、更多地诱导蛋白质成核,并通过调控溶液中蛋白质聚集行为来加速晶体生长。

Six kinds of monomers modified porous SiO2 spheres were designed and synthesized,and the influence of the hydrophilic and hydrophobic surfaces on the aggregation and crystallization of lysozyme protein were systematically studied through DLS and AFM techniques.The experimental results showed that the porous structure could facilitate the disaggregation of the protein ＂clusters＂ and prevent them from hindering crystal growth.Furthermore,this process was enhanced by hydrophobic surface of the porous material,resulting in the monodisperse state of aggregation and subsequent crystallization in a relatively short time,while the hydrophilic surface had the opposite effect.Moreover,the SEM results exhibited that the hydrophobic surface could induce nucleating more and faster,and also accelerate crystal growth by regulating the protein aggregation in solution,compared to the hydrophilic surface.