好氧反硝化菌对高氨氮工业废水脱氮的研究

Research of Aerobic Denitrifier for Treating High Strength Ammonia Nitrogen Industrial Wastewater

从华强化工水处理系统A/O工艺的好氧池中选取污泥,经接种、分离纯化、筛选出了1株在好氧条件下具有良好反硝化效果的菌株B3。按照控制变量法,采取摇瓶实验研究了C/N、DO和pH对好氧反硝化菌株的影响,发现B3菌进行好氧反硝化作用的最优C/N为15,DO为2.3mg/L,pH值为7.0。在好氧条件下能有效去除溶液中NO_3^--N,脱氮率可接近90%。在处理高氨氮废水的性能测试实验中,经过48h的培养,NH_4^+-N、TN、COD去除率分别达到96.9%、78.0%和70.5%,证明B3菌拥有良好的异养硝化和好氧反硝化性能,在处理高氨氮废水和生活污水方面具有广阔的应用前景。当溶液中NH_4^+-N浓度高达700mg/L时,对B3菌脱氮能力的抑制作用明显。

An excellent aerobic denitrification bacterial strain named B3 was achieved by seeding,isolation and purification of the sludge which was taken from the anoxic-aerobic（A/O）process of Huaqiang industrial wastewater treatment system.Through the flask experiment and control variable method,the effects of C/N ratio,DO and pH on the performance of aerobic denitrification were studied and the experimental results demonstrated that the optimal C/N ratio,DO and pH were 15,2.3mg/L and 7.0,respectively.The NO3^--N in the culture could be efficiently removed with the removal rate about 90% and the removal efficiencies of NH＋4-N,TN,and COD were 96.9%,78.0%,and 70.5% after 48 hours of treating high strength ammonia wastewater by bacterial strain B3 under aerobic condition which in turn proved that the stain had a good heterotrophic nitrification-aerobic denitrification capability.Besides,the inhibition phenomenon was obviously found when the concentration of NH4^＋-N was up to 700 mg/L.Therefore,it could be inferred that the stain B3 had broad application prospects in the field of treating domestic and high concentration nitrogen industrial wastewater.