摘要
空肠弯曲菌(Campylobacter jejuni-S_(131),CJ-S_(131))免疫小鼠建立诱导型狼疮样模型。模型鼠脾细胞加入CJ-S_(131)再次刺激以建立离体炎症模型,同时离体给予小叶黑柴胡多糖(Bupleurum smithii var.parvifolium polysaccharides,BPs),研究BPs防治狼疮的作用机制。BALB/c小鼠随机分为正常对照组、佐剂对照组和模型组。在Day 0和Day 14,以CJ-S_(131)免疫小鼠建立狼疮样模型。Day 19取各组脾细胞,再分为空白组、单给药物组(BPs 5、10、20和40μg·m L^(-1))、CJ-S_(131)单刺激组和CJ-S_(131)刺激剂联合药物处理组。ELISA法检测培养48 h上清中总免疫球蛋白G(Ig G)、抗双链DNA(ds DNA)抗体、干扰素-γ(IFN-γ)、白细胞介素^(-1)0(IL^(-1)0)和IL-17的水平,MTT法测定药物对狼疮样小鼠脾细胞增殖活性的影响。结果显示,BPs显著地降低CJ-S_(131)离体再刺激引起的总Ig G、抗ds DNA抗体、IFN-γ和IL-10的高表达。BPs对高表达的IL-17水平和脾细胞活性无明显影响。研究表明,CJ-S_(131)离体再刺激狼疮样小鼠脾细胞可建立离体炎症模型。
Mice were immunized with Campylobacter jejuni-S131(CJ-S131) to establish the lupus-like model. Splenocytes from lupus like mice were challenged with CJ-S131 to induce inflammatory response in vitro. Bupleurum smithii var. parvifolium polysaccharides(BPs) was added in the inflammatory model to observe its underlying mechanisms of action on lupus. BALB/c mice were randomly divided into three groups including normal control group, adjuvant control group and lupus-like model. Mice were immunized on Day 0 and 14 with CJ-S131 to establish lupus-like syndrome, and sacrificed on Day 19. Splenocytes from each group were collected and divided into blank control group, BPs added group(BPs 5, 10, 20, 40 μg·m L^-1), CJ-S131 stimulated group, and CJ-S131 plus BPs group. The levels of total Ig G, anti-ds DNA antibody, interferon-γ, interleukin-10(IL-10) and IL-17 were quantified by ELISA. The proliferation of splenocytes was determined in the MTT assay. BPs significantly suppressed the high levels of total Ig G, anti-ds DNA antibody, IFN-γ and IL-10 stimulated by CJ-S131 and had no significant effects on increased IL-17 secretion and splenocytes proliferation. The results suggest that re-stimulation of splenocytes with CJ-S131 could establish an inflammatory model in vitro. The effect of BPs on lupus might is related to its inhibition of the production of autoantibodies and associated cytokines.
出处
《药学学报》
CAS
CSCD
北大核心
2016年第7期1098-1104,共7页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(81274165)
上海市科委基础研究重点项目(12JC1400800)