补体C3f去精氨酸片段对系统性硬化症小鼠皮肤纤维化模型构建及转化生长因子β1表达的影响

Effection of DRC3f on the dermatofibrosis and TGF-β1 expression of systemic sclerosis mice

目的探讨补体C3f去精氨酸片段(DRC3f)对于系统性硬化症(SSc)小鼠皮肤纤维化模型构建和转化生长因子(TGF)-β1表达的影响。方法采用DRC3f联合博来霉素皮下注射的方法构建SSc小鼠模型,并用纤维染色和免疫组化方法比较联合与不联合DRC3f对SSc小鼠皮肤纤维化、皮肤增厚程度和TGF-β1表达的影响。结果联合DRC3f和博来霉素注射组与单用博来霉素和博来霉素联合无关多肽组比较,皮肤纤维化程度更加明显,TGF-β1的表达也明显增多(P<0.05)。结论 DRC3f能够促进SSc模型小鼠皮肤纤维化和炎症因子TGF-β1的表达。

Objective To investigate the effection of DRC3 f in the dermatofibrosis and TGF-β1 expression of systemic sclerosis mice. Methods The systemic sclerosis mice model was made by hypodermic of DRC3 f combined with Bleomycin. The expression of TGF-β1and the level of dermatofibrosis were detected by immunohistochemistry and fibro-stain respectively. Results The level of dermatofibrosis and the expression of TGF-β1 were much higher in the group which was injected of DRC3 f combined with Bleomycin. Conclusions The DRC3 f might probably promote the dermatofibrosis and the expression of TGF-β1 of systemic sclerosis mice.