胀果甘草多糖对小鼠脾淋巴细胞增殖及诱生细胞因子的影响

Effect of Polysaccharide from Glycyrrhiza inflata on Spleen Lymphocytes Proliferation and Induction of Cytokine in Mice

目的：研究胀果甘草多糖Gi P-B1对小鼠脾淋巴细胞增殖及诱生IL-2、IL-13、TNF-α等细胞因子的影响。方法：采用磺酰罗丹明B（SRB）法检测不同浓度Gi P-B1单独作用及其与有丝分裂原Con A、LPS共同作用对小鼠脾淋巴细胞增殖的影响;采用酶联免疫吸附实验（ELSA）法检测不同浓度Gi P-B1体外刺激对小鼠脾淋巴细胞诱生白介素-2（IL-2）、白介素-13（IL-13）、肿瘤坏死因子-α（TNF-α）等细胞因子的影响。结果：Gi P-B1在25~100μg/m L浓度范围内能显著促进小鼠脾淋巴细胞增殖,并与Con A、LPS协同促进T、B淋巴细胞增殖,还能诱导脾淋巴细胞分泌l L-2、TNF-α。结论：Gi P-B1具有免疫增强作用。

Objective： To investigate the effect of an acidic polysaccharide from Glycyrrhiza inflata（ Gi P- B1） on spleen lymphocytes proliferation and induction of cytokine in mice. Methods： The effects of treatments with Gi P- B1 and with Gi P- B1 containing motigen at different concentrations on spleen lymphocyte proliferation were determined by SRB.The contents of IL- 2,IL- 13 and TNF- α were determined by ELISA. Results： Gi P- B1 at a concentration ranging from25 μg / m L to 100 μg / m L resulted in a significant increase of lymphocyte proliferation. Gi P- B1 could also enhance the effect of Con A- induced T lymphocyte proliferation and the stimulation index of LPS- induced B lymphocytes. Moreover,Gi P- B1 could increase the secretion of IL- 2 and TNF- α in spleen lymphocyte. Conclusion： Gi P- B1 has the effect on enhancing immune responses in mice.