摘要
RNA干扰(RNA interference,RNAi)是真核生物中高度保守的,由小分子干扰RNA(small interfering RNA,si RNA)介导的转录后基因沉默现象,在基因功能的研究中得到了非常广泛的应用,并有望成为小核酸药物在疾病治疗中发挥重要作用。现对近年来RNAi的作用机制、si RNA的产生途径、引起RNAi副作用的原因以及RNAi表达载体的设计这四个方面的国内外研究进展进行了总结,对RNAi与CRISPR技术在应用中的关系进行了探讨,展望了RNAi技术未来的发展。
RNA interference(RNAi) is an evolutionarily conserved post-transcriptional gene silencing mechanism mediated by small interfering RNA(si RNA) in eukaryotes, which has become a powerful tool to silence gene and holds great promising for combating human diseases. This review summarizes the current knowledge about the mechanism of RNAi, off-target effects and designing of si RNA-expressing vectors. We also compare the RNAi with CRISPR technology and further discuss the prospect of its future applications.
出处
《生命科学》
CSCD
2016年第5期576-583,共8页
Chinese Bulletin of Life Sciences
基金
国家自然科学基金面上项目(31470781)