热处理程度对厌氧颗粒污泥发酵产氢性能的影响

Effect of Different Heat Pretreatment Times on Fermentative Hydrogen Production from Anaerobic Granular Sludge

为寻求适宜的种泥热处理时间,加速生物发酵制氢系统的启动进程并提高产氢效能,以易得的厌氧颗粒污泥为研究对象,通过间歇发酵试验,探讨了污泥在95℃热处理温度、不同处理时间条件下的发酵葡萄糖产氢特性。摇瓶发酵试验表明,在初始pH值为7.0、葡萄糖浓度为10 000 mg/L、污泥接种量为2g/L等条件下,由经过不同热处理时间的颗粒污泥构建的发酵系统中,其发酵产氢量有较大不同。其中,经过45 min热处理的污泥具有更高的发酵产氢性能,在144h的发酵过程中,其累积产氢量为163.72 mL,比产氢率为19.62 mmol//gMLVSS,葡萄糖的氢气转化率达到1.72 mol/mol。颗粒污泥发酵葡萄糖的液相末端产物均以乙醇、乙酸和丁酸为主。其中,丁酸的含量接近50%,表现为丁酸型发酵。

In order to find appropriate heat pretreatment time to speed the start-up of fermentative hydrogen production system and improve the efficiency of hydrogen production, the characteristics of fer- mentative hydrogen production from glucose at 95℃ with different pretreatment times were investigated in batch cultures with accessible anaerobic granular sludge. The results showed that under the conditions of initial pH of 7.0, glucose concentration of 10 000 mg/L and seed sludge volume of 2 g/L, the hydrogen production was different in the seed sludge fermentation system formed by anaerobic granular sludge heat- ed at different times. The sludge with heat pretreatment for 45 min had a higher fermentative hydrogen production. The maximum accumulative hydrogen production of 163.72 mL, specific hydrogen production rate of 19.62 mmol/gMLVSS and hydrogen yield of 1.72 mol/mol glucose were achieved during the fer- mentation period of 144 h. Ethanol, butyrate and acetate were dominated in soluble liquid products for the glucose fermentation tests, and the account of butyrate was about 50% of the total volatile fatty acids, showing butyrate-type fermentation.